针刺对多因素致慢性疲劳大鼠HPA轴功能影响的实验研究_曲长江.pdfVIP

辽宁中医杂志 2010年第 37卷第 10期 # 2055# HPA , , , (辽宁中医药大学, 辽宁 沈阳 110032) : 目的: 探讨针刺干预对多因素致慢性疲劳大鼠HPA 轴相关激素的影响方法: 采用多因素(束缚 + 泳+ 昼夜颠倒 + 空瓶刺激)的方法建立慢性疲劳大鼠模型针刺组大鼠每日针刺足三里太冲百会共 21天, 观察针刺干预 对多因素致慢性疲劳大鼠模型血清 CORTACTH下丘脑 CRHmRNA 的影响结果: 针刺可以明显提高模型大鼠体重 体温; 缓解模型大鼠体力疲劳, 提高学习记忆能力, 改善模型大鼠情绪的异常模型大鼠经治疗后血清 CORT 和ACTH 含 量较模型组明显降低; 下丘脑 CRHmRNA含量下降, 接近正常水平说明针刺具有调节慢性疲劳大鼠的 H PA轴功能紊 乱, 抑制H PA轴过度兴奋的作用结论: 针刺百会足三里太冲穴具有明显改善慢性疲劳大鼠疲劳状态和降低 H PA轴 兴奋性的作用, 是治疗 CFS的有效方法 : 慢性疲劳综合征; CORT; ACTH; CRHmRNA; 应激 : R332 : B : 1000- 1719( 2010) 10- 2055- 03 ExperimentalResearch ofEffects ofAcupuncture on theHPA Axis of ChronicFatigueRats Induced byMultiple Stress Factors Q U Chang-j iang, X IE Kun, QU J ing, LIU Bai-lu (L iaoning University of Ch inese M ed i ine, Shenyang 110032, Liaoning, China) Abstract:Obj e tive: To d isc ss the effects of ac p nct re on the relative horm ones of theHypothalam ic- P it itary- Adrenal ( HPA) axis of chron ic fatig e rats ind ced by m ltiple stress factors.M ethods: It is th ro gh m ltip le stress factors of constraint, sw mi m ing, day- night reversal and em pty bottle stmi lation to set p the chron ic fatig em odel of rats. And rats in the ac p nc- t re gro p are p nct red in b ilateral Z sanl,i Taichong, Baih i once a day for 21 days. Then the effects of ac p nct re on the con- tents of the CORT and ACTH in ser m and the CRHmRNA in hypothalam s of ch ronic fatig e rats are observed. Results: After ac- p nct re treatment, the body weight and temperat re of rats in the ac p nct re gro p increase, the ab ility of spatial learn ing and m em ory, the physical fatig e and the changes of em otion are mi proved. And