活性氧对雨生红球藻生长和虾青素含量的影响.pdfVIP

生 态 科 学 2006 年 6 月 第 25 卷 第 3 期 ECOLOGIC SCIENCE Jun., 2006, 25(3):213～215 活性氧对雨生红球藻生长及虾青素含量的影响 * 王 劲，段舜山 （暨南大学水生生物研究所，广州石牌 510632 ） 【摘要】 在雨生红球藻培养液中分别添加活性氧 1O 、H O 和·OH 的诱生剂，通过测定细胞密度、叶绿素a 含量、虾青 2 2 2 素含量，研究了这三种活性氧诱生处理对雨生红球藻生长和虾青素含量的影响，初步探索了利用活性氧诱生剂提高雨生红 -7 -1 球藻虾青素含量的可行性。实验结果表明，适当浓度的 MB 能够促进虾青素含量增加，当MB 浓度为 10 mol ·L 时，虾 青素含量达到 5.27μg ·mL-1 ，比对照显著提高。活性氧诱生剂对雨生红球藻生长有抑制作用，但 MB 的抑制作用小于H O 2 2 和·OH 诱生剂。 关键词： 雨生红球藻；活性氧；虾青素 中图分类号：Q949.2 文献标识码：A 文章编号：1008-8873(2006)03-213-03 Impact of active oxygen reagents on growth and astaxanthin content in Haematococcus pluvialis WANG Jin, DUAN Shun-shan (Hydrobiological institute, Jinan University, Guangzhou 510632) Abstract By determining the cell quantity, content of chlorophyll-a, astaxanthin content in Haematococcus pluvialis, the effects of three reactive oxygen species 1O 、H O and ·OH on the growth and the accumulation of astaxanthin content were 2 2 2 investigated,, preliminarily seek to the feasibility to enhance the astaxanthin biosynthesis by ROS. The results showed that 1) the content of asataxanthin in Haematococcus pluvialis was obviously increased by proper concentration of MB. When the concentration of MB was 10-7 mol ·L-1, content of asataxanthin could reach to 5.27μg ·mL-1, much higher than the control. 2) the growth of Haematococcus pluvialis was restrained by the ROS, but the restraining effect of MB was not so seri