Prokaryotes and bacterial growth curve.ppt

Prokaryotes and bacterial growth curve Domains of life Domains of life Bacteria and Archea Bacteria and Archea are unicellular organisms. For long time, Archea used to be grouped with bacteria but it was separated later on. Generally Archea can be described as bacteria able to grow and multiply in extreme environmental conditions (high temperature, high salinity,….) Why to study Bacteria and Archea Many of them produce diseases (HUS caused by E. coli O157). Others are beneficial (lactobacillus species is used in yogurt, bacteria in association with plant roots supply nitrogen). Archea is good source for restriction enzymes Bacterial morphology Bacteria has many shapes B.subtilis is bacilli (rod shaped) Bacterial reproduction Simple binary fission (Asexual) The bacterium double its content then divide into two Bacterial motility /~skuo/emph_ListSteps.html#Relevance Bacterial growth curve Bacteria grow in population called Culture Binary fission result in “geometric progression in number” which is termed exponential growth 1- 2- 4- 8-16 - 2n…. Generation time (doubling time):time needed for division Growth curve How to calculate generation timefrom data set How to calculate generation time How to calculate generation time What to do How to draw a growth curve at the exponential phase How to calculate generation timefrom a growth curve Microscope Lab report on bacteria and bacterial growth curve * This lab Bacteria, Archea Bacterial morphology, reproduction and growth curve How to calculate generation time How to draw a growth curve Exercise on phase contrast microscope Inoculating some plates DNA RNA ATP Chlo. AB Chlo.A Muramic acid Blue green bacterium chloroxybacteria schizomycetes Archaea Eukarya DNA transcription factors Nuclear mem. Intracellular organelles n = 3.3 x log (P/p) n= number of generations P= final population number p= original population number Generation time = time elapsed to reach “P” minute/generation