市售配方粉中阪崎肠杆菌检测方法的比较.pdfVIP

210 2008 20 3 CHINESE JOURNAL OF FOOD HYGIENE 论著 裴晓燕郭云昌余东敏刘秀梅 ( 中国疾病预防控制中心营养与食品安全所, 北京100050) : 比较和分析两种不同的 崎肠杆菌定性检测方法的区别修改采用美国FDA 和 ISO 崎 肠杆菌检测方法, 同时对194 份市售配方粉进行定性检测 经过选择性增菌和分离, 第二法中待鉴定的 崎肠杆菌疑似菌落只有8 个, 明显少于第一法, 而且其特征性菌落易于辨认运用两种方法分别检测到4 个和5 个 阳性样品, 其中第二法检出的1 个阳性样品在第 一法中未检出 第二法中的选择性增菌液肉汤mlST- Vm 和 崎肠杆菌显色培养基的选择性明显优于第一法中相应的培养基, 可以大大减少工作量, 简单省时 : 肠杆菌; 婴儿配方; 食品; 微生物技术 Comparison on Detection Methods for Enterobacter sakazakii in Infant Formula Powder from Retail Markets PEI Xiao-yan, GUO Yun-chang, YU Dong-min, LIU Xiu-mei ( National Institute for Nutrition and Food Safety, Chinese CDC, Beijing 100050, China) Abstract: Objective To compare and analyze two different detection methods for E nterobacter sakazakii in infant formula powder. Method According to the detection methods of FDA and ISO for Enter obacter sakazakii , the pathogens from 194 formula powder sampled from the markets were isolated and identified. ResultsAfter selecti e enrichment and isolation, there [ 7] DE MELO ABREU S, ALVES A, OLIVEIRA B, et al . Determination [ 13] , , , . of ethyl carbamate in alcoholic be erages: an interlaboratory study to - [ J] . compare HPLC - FLD with GC - MS methods [ J] . Anal Bioanal ,2007, 19( 6) : 492-495. Chem, 2005, 382( 2) : 498-503. [ 14] BEN J C,FRA L J, GOR B, et al. Determination of ethyl carbamate [ 8] MIRZOIAN A, MABUD A. Comparison of methods for extraction of in alcoholic be erages and soy sauce by gas chromatography w