盐胁迫下棉花基因组DNA表观遗传变化MSAP分析_李雪林.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(4): 588−596 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.00588 盐胁迫下棉花基因组DNA 表观遗传变化的MSAP 分析 李雪林1,2 林忠旭 1 聂以春1 郭小平1 张献龙1,* 1 , 430070; 2 , 471003 : , YZ1 , NaCl DNA − 1 NaCl , 200 mmol L− 1 NaCl ; 100~200 , 100 mmol L mmol L− 1 NaCl (methylation-sensitive amplification poly- morphism, MSAP), 100150 200 mmol L− 1 NaCl DNA 38.1%35.2% 34.5%, (41.2%), DNA NaCl (r = −0.986) , 100150 200 mmol L− 1 NaCl DNA 6.4%7.6% 11.3% 12.7%11.1%8.2%, RT-PCR , MSAP : ; ; DNA ; ; RT-PCR MSAP Analysis of Epigenetic Changes in Cotton (Gossypium hirsutum L.) under Salt Stress 1,2 1 1 1 1,* LI Xue-Lin , LIN Zhong-Xu , NIE Yi-Chun , GUO Xiao-Ping , and ZHANG Xian-Long 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2 College of Agronomy, Henan University of Science and Technology, Luoyang 471003, China Abstract: Salinity is one of the important limiting factors in plant production worldwide. The objectives of the study were to as- sess the effect of salt stress on the plant growth and to determine if DNA can be methylated in cotton plants (Gossypium hirsutum) by methylation-sensitive amplified polymorphism (MSAP) technique. The results showed that 100 mmol L− 1 NaCl obviously promoted plant height and root length of cotton seedlings, but 200 mmol L− 1 NaCl significantly inhibited plant growth; 100–200 mmol L− 1 NaCl inhibited the number of lateral r