肾源性细胞因子促红细胞生成素对急性脊髓损伤大鼠细胞凋亡的影响_陈淑强.pdfVIP

中国组织工程研究与临床康复 第 12 卷 第 11 期 2008 –03 –11 出版 基础医学 Journal of Clinical Rehabilitative Tissue Engineering Research March 11, 2008 Vol.12, No.11 肾源性细胞因子促红细胞生成素对急性脊髓损伤大鼠细胞凋亡的影响**★ 1 1 1 1 1 2 陈淑强 ，徐又佳 ，俞 晨 ，郝彦明 ，张振东 ，顾永平 Protective effect of erythropoietin on neurocyte apoptosis following experimental acute spinal cord injury in rats 1 1 1 1 1 2 Chen Shu-qiang , Xu You-jia , Yu Chen , Hao Yan-ming , Zhang Zhen-dong , Gu Yong-ping Abstract 1Department of AIM: Erythropoietin, a cell factor produced by kidney, can protect spinal nerve from acute spine cord injury (SCI) in rat. The Orthopedics, Second experiment explored the effects of erythropoietin on the apoptosis of neural cells. Hospital of Soochow METHODS: The experiment was performed in the animal laboratory of Second Affiliated Hospital of Soochow University from University, Suzhou 215004, Jiangsu January to April 2007. ①Forty-eight adult male Sprague-Dawley rats of (270 ±10) g were randomly divided into normal group (n Province, China; =6), sham operation group (n =6) underwent laminectomy procedure only, saline group (n =18) and treatment groups (n =18). The 2Teaching and SCI models were established using the improved Allen method. The rats in treatment group were epidurally injected with 5 000 u/kg Research Section of body mass of recombinant human erythropoietin (Kirin Beer Company, Japan, 3 000 IU per piece) 1, 6, and 24 hours after injury Pathology, Department of Basic respectively (6 animals