蔷薇科一些植物鲜叶提取物清除DPPH自由基活性的研究.pdfVIP

植物学通报 2004, 21 (1): 74~78 Chinese Bulletin of Botany 蔷薇科一些植物鲜叶提取物清除DPPH 自由基活性的研究 1 2 胡丰林 陆瑞利 1(安徽农业大学森林利用学院 合肥 230036) 2 (安徽农业大学基础科学学院 合肥 230036) 摘要 用二苯基苦基苯肼自由基酶标仪法，对亚热带蔷薇科常见的50 种木本植物鲜叶的自由基清除活 性进行了比较，发现不同属、不同种树木鲜叶的80% 甲醇提取物的自由基清除活性有很大差异，其 -1 . 中苹果属5 种植物在相当于鲜叶浓度为0.5 mg/mL 于37℃下孵育20 min 时，对0.5 mmol L DPPH 自由 基平均清除率达62.4% ，而绣线菊属3 种植物的平均自由基清除率仅11.6%。尖嘴林檎、棣棠、木 瓜、三叶海棠、湖北海棠、木香花、小果蔷薇和黄山花楸等鲜叶有较强的自由基清除活性，它们 在浓度为0.5 mg/mL 时的自由基清除率分别可达85.0%、75.8% 、70.7% 、69.6% 、64.5% 、62.5% 、 61.8% 和61.3% ，显示其有较大的开发潜力。 关键词 蔷薇科木本植物，鲜叶提取物， 自由基清除率，二苯基苦基苯肼自由基 Studies on Scavenging Activities to DPPH Free Radical of Extracts from Fresh Leaves of Some Woody Plants of Rosaceae 1HU Feng-Lin 2LU Rui-Li 1( College of Forest Utilization, Anhui Agricaltural University, Hefei 230036 ) 2( College of Basic Science, Anhui Agricaltural University , Hefei 230036 ) Abstract A DPPH-Microplate assay were made to determine the free radical scavenging activity of the 80% methanol extracts from the leaves of some subtropical woody plants of Rosacece. The results revealed that all the trees tested have different free radical scavenging capacities, and as the raising of extract concentration and the prolonging of incubation time at 37 ℃, their free radical scavenging capacities increased. Among all trees tested, Malus melliana (Hand.-Mazz.) Rehd, Kerria japonica (L.) DC., Chaenomeles sinensis (Thouin) Koehne , Malus sieboldii (Regel) Rehd., Malus hupehensis (Pamp.) Rehd., Rosa banksiae Ait., Rosa cymosa Tratt.and Sorbus amabilis Cheng et Yu showed the stronger free r