be调控对宫颈癌HeLa细胞增殖和凋亡的作用.pdfVIP

·论著· 增殖和凋亡的作用’ 吴维光，孙小丽，陈劾，林仲秋” (中山大学附属第二医院妇科肿瘤专科，广州510120) 【摘要】 miRNAPrecursor 凋亡的作用及可能的机制。方法：利用转染试剂将Pre—miR—hsa—miR-99b 转染宫颈癌HeLa细胞，荧光实时定量PCR方法检测miR-99b表达，四甲基偶氮唑蓝 (M，I’I)法检测细胞增殖能力，流式细胞仪检测细胞凋亡率，Westernblot检测其靶基因 miRNA Precursor的HeLa细胞， CDC-25A蛋白的表达。结果：转染Pre—miR-hsa-miR-99b miR-99b表达增高86．45倍，细胞增殖能力下降，细胞凋亡率增加，同时细胞CDC-25A蛋 白表达降低。结论：上调miR-99b表达可通过降低靶基因CDC-25A表达，抑制细胞增殖， 诱导细胞凋亡，miR-99b可能成为治疗宫颈癌的靶基因。 【关键词】HeLa细胞；miR-99b；增殖；凋亡 E仃bctsofmicroRNA-99bmodulationOil and ofcervicalcancer proIiferationapoptosis HeLacells．Wu Weiguang，SunXiaoli，ChenQing，eta1．DepartmentofGynecologicOncology， theSecond SunYat—sen 5l0l20 AffiliatedHospitalof University．Guangzhou theeffectsof 【Abstract]Objective：Toinvestigate microRNA-99b(miR明b)up—regula— tedon and ofHeLacellsandits proliferationapoptosis possiblemechanism．Methods：Pre．miR． Precursorwas HeLa tmnsfeetion miR一 hsa．miR—99bmiRNA transfeetedinto cellswith agent．The 99b wasdetectedrealtimePCR．Thecellular was M‘17r expression by growthactivityassayedby the wastested Awere flow ofCDC一25 by expression assay．andapoptosis cytometry．neprotein measuredWestern miR．99b inPre．miR—hsa—miR-99bⅡliRNA by blot．Results：Theexpression cells fold．rnle the Precursortransfeetedincreased86．45 cellular decreased．and growthactivity cell ofCDC-25AdecreasedinPre．miR— apoptosisincreased．Furthermore．theproteinexpression