Min基因突变小鼠模型在肠道肿瘤研究中的应用.pdfVIP

HEREDITAS (Beijing) 2008 3 , 30(3): 277―282 ISSN 0253-9772 综 述 DOI: 10.1360/yc-008-00277 Min 盛弘强, 陈俭, 来茂德 , 310058 迄今为止, 肠道肿瘤相关的基因突变小鼠或敲除小鼠大约有 30 多种, Min(multiple intestinal neoplasia)小 鼠是具有肠道多发性腺瘤特征的 Apc 基因突变小鼠, 被认为是当前较为理想的家族性腺瘤性息肉病(FAP) 的研 究模型。APC 基因是 Wnt 途径中重要的抑癌基因, 该途径不仅是动物胚胎发育过程中关键的信号转导途径, 也 对结直肠肿瘤的发生发展起到不同寻常的作用。对 Min 小鼠模型历史、分子遗传学与表型特征、肠道肿瘤与 Wnt 途径异常、抑癌基因甲基化、TGF-β途径和多药耐药基因等内容进行了介绍, 并分析了该小鼠模型在抗结 直肠肿瘤药物研究中的应用和意义。 APC 基因; Min 小鼠; Wnt 基因 Use of Apc(Min/+) mouse model in the studies of intestinal tumors SHENG Hong-Qiang, CHEN Jian, LAI Mao-De Department of Pathology and Pathophysiology, School of Medicine, Zhejiang University, Hangzhou 310058, China Abstract: More than thirty kinds of mutant or knockout mice bearing intestinal neoplasm have been reported up to the pre- sent. Apc (Min/+) mouse holding multiple intestinal neoplasia, provides an appropriate model to evaluate human familial adenomatous polyposis. APC is an important tumor-suppressor gene in the Wnt pathway, which is involved in the pivotal signal transduction cascade in animal embryogenesis and colorectal carcinogenesis. Apc (Min/+) mouse model was pre- sented as aspects of the strain background, genotype/phenotype, divergent canonical Wnt signaling pathway, methylation of tumor-suppressor gene, TGF-β signaling pathway and multidrug resistance gene, etc. This review also introduced the appli- cation and signification of the mouse model in studies of anti-colorectal tumor drugs. Keywords: APC ; Min mouse; Wnt (familial adenomatous 100%FAP “ ” polyposis, FAP) 1721 Menzel Gardner , 1882 Harrison , Turcot 1925 Lockhart