非小细胞肺癌中VEGF与MMP-2的表达及其与肿瘤侵袭转移关系的研究.docVIP

非小细胞肺癌中VEGF和MMP-2的表达及其与肿瘤侵袭转移关系的研究 　　作者：叶晓锋，高平，李金平，张朝林 　　【摘要】 目的 探讨非小细胞肺癌中血管内皮生长因子(VEGF)和基质金属蛋白酶-2(MMP-2)的表达水平以及与非小细胞肺癌侵袭转移恶性表型形成的关系。方法 对60例随访6-64个月的原发性非小细胞肺癌术后肺癌组织石蜡切片，同时取癌旁正常肺组织，采用免疫组织化学SP法进行VEGF和MMP-2表达水平的检测。结果 VEGF、MMP-2阳性表达率分别为68.3% 和65.0%，与癌旁正常肺组织比较差异有统计学意义(P0.01)。与肿瘤大小、临床分期呈正相关，差异有统计学意义(P0.05)。有淋巴结转移、复发转移患者两者的表达强度显著高于淋巴结无转移、无复发转移患者，差异有统计学意义(P0.05)。两者在肺癌组织中的表达与病人年龄、性别、有无吸烟习惯、组织病理类型均无关(P0.05)。结论 VEGF和MMP-2 在非小细胞肺癌组织中的表达均与肿瘤大小、临床分期、有无淋巴结转移、有无复发转移有关。两者在肺癌组织中的表达具有相似性并显著正相关，可作为判断NSCLC中肿瘤细胞侵袭转移力的预见性指标，对判断其恶性程度其有参考价值。 　　【关键词】 非小细胞肺癌;血管内皮生长因子;基质金属蛋白酶-2;转移;免疫组织化学 　　The study of the relationship between the expression of VEGF and MMP-2 and the tumor incursion　 metastasis of non-small cell lung cancer 　　YE Xiao-feng, GAO-Ping, LI Jin-ping, et al. 　　(The Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, China) 　　[Abstract]Objective [Abstract] Objective To probe into the relationship of the expression level of VEGF and MMP-2 and the shaping of tumor incursion metastasis and malignant phenotype for non-small cell.Methods 60 patients were diagnosed as primary non-small cell lung cancer (NSCLC) with clear-cut pathologic diagnosis and competed pathological data and visited for 6-64 months and whose postoperative lung cancer tissue paraffin section and near cancer part normal lung tissue paraffin section were determined for the expression level of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) with immunohistochemistry SP.Results The positive rates of VEGF and MMP-2 were 68.3% and 65.0%, respectively. Comparing with the cancer side normal tissue, there was significant difference (P0.01). The positive expression rate of VEGF and MMP-2 had positive correlation with clinical pathological stage and the size of the tumor (P0.01), and was both significantly higher in patients with lymph node metastasis and recurred cancer than in those without (P0.01). VEGF and MMP-2 were found of no relationship with age, g