泛素26S蛋白酶体途径与显花植物自交不亲与反应.pdf

植物学通报 2006, 23 (2): 197~206 Chinese Bulletin of Botany . . 专题介绍 泛素/26S蛋白酶体途径与显花植物自交不亲和反应 于晓敏 蓝兴国 李玉花* (东北林业大学花卉生物工程研究所 哈尔滨 150040) 摘要 植物的生长和发育离不开短命调控蛋白的有选择性降解, 其中一种重要的降解方式就是泛素/26S 蛋白酶体途径。在这个途径中, 泛素(ubiquitin)和26S蛋白酶体起着至关重要的作用, 需要被降解的蛋白 会通过E1-E2-E3酶接合反应由Ub进行标记, 随后标记蛋白会被26S蛋白酶体识别并降解。自交不亲和反 应也正是通过此途径实现的, ARC1(arm repeat containing 1)和SCFs (skp1-cul1-F-box-proteins)作为E3s分 别在孢子体自交不亲和和配子体自交不亲和反应中起作用。本文综述了就泛素/26S蛋白酶体途径的组成 及其在自交不亲和反应中的作用。 关键词 26S蛋白酶体, 蛋白质水解, 泛素, 自交不亲和性 The Ub/26S Proteasome Pathway and Self-incompatible Re- sponses in Flowering Plants * Xiaomin Yu, Xingguo Lan, Yuhua Li (Research Institute of Flower Biotechnology , Northeast Forestry University , Harbin 150040) Abstract Plant growth and development is generally controlled by selective removal of short-lived regulatory proteins. One major pathway is represented by the ubiquitin (Ub)/26S proteasome pathway. In this pathway, ubquitin and 26S proteasome play essential roles. Ub is attached to proteins des- tined for degradation by a 3-step enzymatic (E1-E2-E3) conjugation cascade, and the resulting Ub- protein conjugates are then recognized and degraded by the 26S proteasome. The arm repeat-con- taining 1 (ARC1) and the skp1-cul1-F-box (SCF) proteins, belonging to the family of E3 Ub ligases, function in both the sporophytic and gametophytic self-incompatible responses, respectively. This review highlights the recent progress toward understanding the role of the Ub/26S proteasome pathway during self-incompatible responses. Key words 26S proteasome, proteolysis, ubiquitin, self-incompatibility 新多肽的形成和前体蛋白的水解控制着植 些在应激状态下产生的蛋白质和那些通过内吞 物生活的所有方面。植物的生长和发育必须 作用从胞外摄取的蛋白质等主要经溶酶体降解; 选择性地降解一些短命调控蛋白。目前已知 而泛素/ 蛋白酶体途径则是高选择性地进行细 主要有2种不同的蛋白酶解系统控制着真核细 胞内蛋白转换, 尤其是短命的功能蛋白。在泛 胞内的蛋白质降解, 即溶酶体途径和泛素/蛋白 素/蛋白酶体