基于液质联用数据库技术的中药和天然产物化学成分快速鉴定方法的建立.pdfVIP

[2] 王曼华，孙化萍，梁建卫. 经方―药对‖配伍理论研究概况[J]. 辽宁中医药大学学报，2008，10 （1） [3] 李志青. 相近药名对药的配伍应用举隅[J]. 山西中医学院学报，2009 ，10 （6）：42-43. [4] 郭辉，张玲. 乳香中化学成分和药理作用的研究进展[J]. 食品与药品，2007，9 （5 ）：50-52. [5] 沈涛，娄红祥. 没药的化学成分及其生物活性[J]. 天然产物研究与开发，2008 ，20 ：360-366. （以下省略） Analyzing the chemical components of frankincense-myrrh herbal pair by UPLC/Q-TOFMS Chen Ting, Su Shulan, Duan Jin-ao, Shang Erxin, Qian Dawei, Tang Yuping (Jiangsu Key laboratory for TCM formulae Research, Nanjing University of Chinese Medicine, Jiangsu Nanjing 210046) Abstract: In the present study, an ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOFMS) based on chemical profiling approach to evaluate chemical constitution between mixed decoction and co-decoction of frankincense-myrrh, reveal the synergistic material basis after the compatibility of frankincense-myrrh. Two different kinds of decoctions, namely frankincense-myrrh co-decoction: water herbs mixed together, were prepared. Batches of these two kinds of decoction samples were subjected to UPLC/Q-TOFMS analysis, the datasets were processed with MassLynx 4.1 to holistically compare the difference between these two kinds of decoction samples. The most changed components during decocting were analyzed. Using the proposed approach, global chemical difference was found between co-decoction and mixed decoction, pentacyclic triterpenoid compounds (α-boswellic acid, β-boswellic acid) and tetracyclic triterpenoid ( elemonic acid,3-acetoxy-16-hydroxy-dammar-24-ene,3-hydroxytirucalla-8,24-dien-21-oicacidor3-hydroxytirucalla-7,24-dien -21-oic acid) compounds were identified as the most changed components (changed most significantly) during decocting. The correlation between components and bio-effect must be research further. This paper may provide a