低氧诱导因子1病毒载体抑制海马神经元凋亡：基因治疗前途.docVIP

低氧诱导因子1病毒载体抑制海马神经元凋亡：基因治疗的前途 由于缺氧诱导因子1α可作为一种神经保护因子，防止神经细胞凋亡，因此中国河北化工医药职业技术学院柴锡庆教授所在团队设计了一项研究，以表达低氧诱导因子1α的重组腺病毒载体转染原代培养的海马神经元细胞，并在大鼠海马内高效表达低氧诱导因子1α蛋白，抑制了海马神经细胞凋亡。作者认为，其机制可能与低氧诱导因子1α降低海马神经元内游离钙浓度，从而抑制凋亡级联反应有关。此项研究为今后应用表达低氧诱导因子1α的重组腺病毒载体进行阿尔茨海默病基因治疗提供了实验依据。相关文献发表于《中国神经再生研究（英文版）》杂志2014年6月第11期。 电镜观察显示转染了表达低氧诱导因子1α的重组腺病毒载体的海马神经元经淀粉样β蛋白诱导后凋亡现象明显减轻 Article: A viral vector expressing hypoxia-inducible factor 1 alpha inhibits hippocampal neuronal apoptosis, by Xiqing Chai1, Weina Kong1, Lingyun Liu2, Wenguo Yu1, Zhenqing Zhang3, Yimin Sun1 (1 Bioreactor and Protein Drug Research and Development Center of Hebei Universities, Hebei Chemical and Pharmaceutical College, Shijiazhuang, Hebei Province, China; 2 Department of Neurology, Shanghai Yangpu District Central Hospital, Shanghai, China; 3 Department of Neurology, the First Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China) Chai XQ, Kong WN, Liu LY, Yu WG, Zhang ZQ, Sun YM. A viral vector expressing hypoxia-inducible factor 1 alpha inhibits hippocampal neuronal apoptosis. Neural Regen Res. 2014;9(11):1145-1153. 欲获更多资讯： Neural Regen Res HIF 1α viral vector inhibits hippocampal neuronal apoptosis: the future of gene therapy Hypoxia-inducible factor 1 (HIF-1) attenuates amyloid-beta protein neurotoxicity and decreases apoptosis induced by oxidative stress or hypoxia in cortical neurons. Prof. Xiqing Chai and co-workers from Hebei Chemical and Pharmaceutical College, China constructed a recombinant adeno-associated virus (rAAV) vector expressing the human HIF-1α gene (rAAV-HIF-1α) efficiently, and tested the assumption that rAAV-HIF-1α represses hippocampal neuronal apoptosis induced by amyloid-beta protein. Their results confirmed that rAAV-HIF-1α significantly reduces apoptosis induced by amyloid-beta protein in primary cultured hippocampal neurons. This mechanism may be related to HIF-1α-decreased hippocampal neuronal intracellular calcium concentration ([Ca2+]i), thereby inhibiting apoptotic cascade