IL-27促进DC分化成熟及其作用机制的实验研究.pdfVIP

stimulatoryfactorsinthefifthday:Negativegroup,TNF-αgroup (TNF-α:20ng/ml),IL-27group (IL-27: 20ng/ml),TNF-α+IL-27group(co-cytokinesgroup,TNF-α:10ng/ml+IL-27∶10ng/ml).ThemorphousofDC wasobservedbyinvertedmicroscopeduringthe7days.TheexpressionsofCD1aandCD83,CD80andCD86, andICAM-1onDCwereanalyzedbyFACS.TheeffectontheproliferationofTcellsstimulatedbyDCtreated withMitomycinCwasanalyzedbyMTS.ThelevelsofIL-12andIFN-γintheculturesupernatantofDCwere detectedbyELISA.TheexpressionsofMAPKp38,P-STAT1andP-STAT3inDCweredetectedbyWestern-blot. ResultsDCfromIL-27groupandTNF-α+IL-27grouphadatypicalmorphol