人牙周韧带细胞与β磷酸三钙多孔生物陶瓷的牙周组织工程研究.docVIP

人牙周韧带细胞和β-磷酸三钙多孔生物陶瓷的牙周组织工程研究 作者：张翼 王贻宁　赵艳 田敏 陈新民 【关键词】 组织工程化牙周膜 　　［摘要］ 目的：以多孔Β-磷酸三钙（Β-TCP ）生物陶瓷材料作为组织工程支架，以人牙周韧带细胞（PDLCs）作为种子细胞，探索构建组织工程化牙周膜的可行性。方法：将体外培养7 d的PDLCs和Β-TCP的复合物，植入裸鼠背部一侧皮下，在对称部位植入单纯的Β-TCP作为空白对照。术后4周、8周、12周分别取材，进行组织学检测。结果：实验组8周后支架材料部分吸收，可见未降解的支架材料孔洞内广泛分布着新生组织，其内可见小血管、腺体等结构；12周时支架材料大部分吸收，取而代之的是大量片状的新生结缔组织。空白组组织形成较少，支架材料降解与实验组相似。结论：体外培养的PDLCs与Β-TCP复合后，能在体内增殖、分化，形成结缔组织，从而证明以Β-TCP作为支架材料进行的牙周组织工程是可行的。 　　［关键词］ 组织工程化牙周膜 牙周韧带细胞 Β-磷酸三钙 　　A Study of Tissue Engineered Periodontal Ligament Based on Human Periodontal Ligament Cells and Β-TCP. 　　ZHANG Yi, WANG Yi-ning, ZHAO Yan, et al. 　　Key Laboratory of Oral Biomedical Engineering (Wuhan University), Ministry of Education. Wuhan 430079 　　［Abstract］Objective: To investigate the possibility of generate tissue engineered periodontal ligament by using porous Β-TCP as scaffold and human periodontal ligament（PDL） cells as seed cell. Methods: PDLCs were seeded onto scaffolds and implanted subcutaneously in BALB/c-nu mice. The control group were implanted by Β-TCP only. Formed tissues were harvested at 4,8,12 weeks after operation for histological analysis. Results: In the experimental group, histological analysis demonstrated obvious degradation of Β-TCP and extensive new formation at 8 weeks after operation. The degradation of Β-TCP was even obvious at 12 weeks after operation, and the cells on Β-TCP formed sparse monolayers as well as the connected tissues. In the control group, only a small quantity of new formation could be seen. Conclusion: It is feasible to generate tissue engineered periodontal ligament by using porous Β-TCP as scaffold and hPDLCs as seed cell. 　　［Key words］ Tissue engineering Human periodontal ligament cells Β-tricalcium phosphate 骨整合的概念由于其在理论和临床上所取得的巨大成功而获得共识，然而，相对于天然牙，骨整合种植体缺乏牙周韧带这一特殊结构，对受力和位移的感觉比较迟钝，种植体易受创伤。同时由于牙龈与种植体附着远比天然牙的附着脆弱，是界面上的一个薄弱环节［1］。因此，近年来种植体周牙周膜的构建一直是研究的热点。本实验室采取组织工程的概念构建种植体周牙周膜，将牙周韧带细胞作为牙周膜组织工程的种子细胞，以多孔Β-TCP作为支架材料，进行体外三维立体培养后进行体内移植，