牵张力作用下甲状旁腺激素相关蛋白对人成骨样细胞增殖影响.docVIP

牵张力作用下甲状旁腺激素相关蛋白对人成骨样细胞增殖的影响 牵张力作用下甲状旁腺激素相关蛋白对人成骨样细胞增殖的影响 作者：黄生高 钟孝欢 王会欣 王明朗 作者单位：中南大学湘雅二医院 口腔科，湖南 长沙 410011 【摘要】 　目的 研究牵张力作用下甲状旁腺激素相关蛋白（PTHrP）对人成骨样细胞增殖的影响。方法 建立体外培养细胞牵张力施力模型。利用逆转录聚合酶链反应（RT-PCR）法检测PTHrP mRNA的表达及12%牵张应变作用下PTHrP（1 nmol/L）对MG-63细胞c-fos mRNA表达的影响。检测不同浓度PTHrP（0、0.01、0.1、1 nmol/L）及12%牵张应变联合作用12 h后对细胞增殖活性的影响。结果 不同牵张力对PTHrP mRNA表达影响的差异具有统计学意义，12%牵张应变组作用的影响最为显著。牵张力联合PTHrP刺激下培养较PTHrP或牵张力的单独效应具有更强的促MG-63增殖作用。牵张力联合PTHrP作用较牵张力的单独效应具有更强的增加MG-63细胞c-fos mRNA表达的作用。结论 牵张力可显著影响人成骨样细胞PTHrP mRNA的表达水平。PTHrP在牵张力环境下较牵张力的单独效应具有更强的促MG-63增殖作用。在牵张力环境下PTHrP可能通过c-fos信号传导途径影响成骨细胞增殖。 【关键词】 张应力 甲状旁腺激素相关蛋白 增殖 　　Effect of parathyroid hormone related protein on proliferation of human osteoblast-like cell under tensionforce HUANG Sheng-gao, ZHONG Xiao-huan, WANG Hui-xin, WANG Ming-lang. （Dept. of Stomatology, Xiangya Second Hospital, Central South University, Changsha 410011, China） 　　[Abstract] Objective To investigate the effect of parathyroid hormone related protein（PTHrP） on proliferation of human osteoblasts（MG-63） under the circumstance of tension force in vitro. Methods An apparatus was designedand fabricated by which force was loaded onto the cultured cells in vitro. Reverse transcription-polymerase chain reaction（RT-PCR） was used for measuring the expression of PTHrP mRNA and c-fos mRNA. The effect of tensionforce and different PTHrP dose（0, 0.01, 0.1, 1 nmol/L） on the proliferation of human osteoblasts were examined usingflow cytometry. Results Various forces of the mechanical stretching exerted different influences on the intensities of the mRNA′ expression. The strain of 12% induced the most remarkable mRNA′ expression. The mitogenesis happened in the group with tension force（12%） combined with PTHrP was more active than that in the group withPTHrP or tension force only. Tension force combined with PTHrP induced significantly more c-fos mRNA than that of tension force only. Conclusion The mechanical stretching can inevitably influence