不同生长年限滇重楼HPLC指纹图谱地的研究_夏从龙.pdfVIP

·专 栏 · ·中药与天然药 · 不同生长年限滇重楼 HPLC 指纹图谱的研究 (大理学院药学院，云南 大理 671000) 摘要：目的 方法 结果 6 结论 关键词： 中图分类号：R917.101 文献标志码：A 文章编号：1007-7693(2011)06-0515-05 HPLC Fingerprints of Paris Polyphylla Smith Var. Yunnanensis of Different Growing Years XIA Conglong, ZHAO Jie, LÜ Shuangshuang, WU Siwen, ZHANG Ye(College of Pharmacy, Dali University, Dali 671000, China) ABSTRACT: OBJECTIVE To compare the fingerprints of Paris polyphylla Smith var. yunnanensis of different growing years and explore its optimal harvesting year. METHODS Paris polyphylla Smith var. yunnanensis samples harvested in different years were collected. The fingerprints of the samples were generated by HPLC. The similarity between samples with different growing year, as well as the chromatographic data, were computed and analyzed. RESULTS The fingerprints of Paris polyphylla Smith var. yunnanensis were influenced by the different of growing years. And Paris polyphylla Smith var. yunnanensis grawing in the sixth year was verified to contain higher content to biological substance. CONCLUSION The data will be very important guide the harvest of Paris polyphylla Smith var. yunnanensis. KEY WORDS: Paris polyphylla Smith var. yunnanensis ; HPLC; fingerprint 滇 重 楼 为 百 合 科 植 物 云 南 重 楼 Paris 充分利用提供参考。 polyphylla Smith var. yunnanensis (Franch.) Hara 的 1 仪器与试药 干燥根茎[1] 。现代药理研究表明，该药具有抗肿瘤、 Agilent 1200 高效液相色谱仪(美国 Agilent 公 抗菌消炎、止血、镇静镇痛、抑制精子活性、抗 司) ；KQ-250B 型超声波清洗器( 昆山市超声仪器有 细胞毒、免疫调节等广泛的药理活性[2] ，应用较广。 限公司) ；RE-2000 型旋转蒸发仪(上海亚荣仪器有 重楼又是一些著名中成药如云南白药、宫血宁胶 限公司) ；AE240 天秤[梅特勒-托利多仪器(上海) 囊、楼莲胶囊