条锈菌诱导的小麦bZIP转录因子基因的克隆和表达分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2010, 36(7): 1221−1225 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.01221 条锈菌诱导的小麦bZIP 转录因子基因的克隆及表达分析 张 毅1 夏 宁1 张 岗1 郭 军1 黄丽丽1 康振生 1,2,* 1 , 712100; 2 , 712100 : RT-PCR , 11 cDNA bZIP cDNA , TabZIPTabZIP 1 071 bp , 356 , bZIP ; bZIP ; TabZIP , ; , TabZIP , ; , TabZIP : ; ; bZIP ; ; Cloning and Expression Analysis of a bZIP Transcription Factor Gene in Wheat Induced by Stripe Rust Pathogen 1 1 1 1 1 1,2,* ZHANG Yi , XIA Ning , ZHANG Gang , GUO Jun , HUANG Li-Li , and KANG Zhen-Sheng 1 College of Plant Protection, Northwest AF University, Yangling 712100, China; 2 Shaanxi Provincial Key Laboratory of Molecular Biology for Agriculture, Northwest AF University, Yangling 712100, China Abstract: In plant, basic leucine zipper (bZIP) transcription factors play various roles in developmental processes and in response to biotic and abiotic stimuli. In the present study, a novel bZIP gene, designated as TabZIP, was isolated from wheat leaves in- fected by Puccinia striiformis f. sp. tritici using in silico cloning and reverse transcription PCR approaches. TabZIP was predicted to encode a 356 amino-acid protein, which contained a bZIP transcription factor basic domain signature and a leucine zipper motif. Multiple alignment analysis based on the amino acids encoded by different bZIP genes from rice (Oryza sativa), maize (Zea mays), Arabidopsis thaliana , indicated that TabZIP was conserved among the three species of plants with h