人喉癌细胞异质性的研究.pdfVIP

42 ·基础研究 · 1【】同医疗前沿 2011年2月 第6卷 第4期 NationalMedica1FrontiersofChina，Feb．2010Vol6No．4 人喉癌细胞异质性的研究 靳阳子 皇甫辉 郭梦媛 【摘要 】目的 通过原代培养人喉癌实体瘤细胞，克隆分离异质性细胞亚群，初步研究喉癌细胞肿瘤异质性、方法 原代培养人 喉癌细胞，应用有限稀释法对体外培养的喉癌细胞进行单细胞克隆分离异质性亚群，比较其在形态、分化能力、细胞周期、增 殖能力及裸鼠异体移植成瘤能力等方面的差异。结果 分离到1型和2型细胞形态差异最大的两个亚群。1型细胞成长梭形， 突起少，亚克隆培养未产生子代细胞，裸鼠异体移植不成瘤 (0／5)；2型细胞成多角形，多突起，继续培养产生的子代克隆出现异 质性细胞 ，裸鼠异体移植可成瘤 (5／5)，体外增值能力强，处于细胞周期G ／G 的比例明显高于1型细胞。、结论 原发性人喉癌实 体瘤细胞具有异质性，2型细胞具有肿瘤干细胞的特性。 【关键词 】喉癌实体瘤 ；异质性 ；肿瘤干细胞 doi：10．3969／j．issn．1673—5552．2011．04．0021 【中图分类号 】R739．65 【文献标识码 】A 【文章编号】1673—5552(2()11)()4一())【42一()2 Research on heterogeneity ofhuman laryngealcarcinoma HN Y3ng—zi。HUANGFU Hui。GUO M eng—yuan． DepartmentofOtolaryngology，FirstClinicalMedicalCollege，ShanxiMedicalUniverdty，Taiyuan DDD』，China [AbstractlObjectiveTo selection cancerstem cell-likesubgroup from heterogeneoussubpopulation cellsin human laryngealcarcinoma．Methods Laryngealcarcinoma cellswere cultured in vitro by primary tissueculture technique． }1eterOgeneOus subpopulatiOns were isolated by limiting dilution and were identified their identified their functional status(mRNA)，cellcycles(G0／G】)andmorphology．Eachsubpopulationwasimplanted in nudemicesubcutaneouslyto measurethetumorformingability．ResultsTwo subpopulationsoflaryngealcarcinoma cellswerederived from primary tumor tissues，type 1and type-2．Type-l cells showed a spindle feature and could not produce new tumor when ireplantedinnudemice(0／5)．However．type-2cellsweremultangularinshapewithprotuberancesandcouldform new tumor(5／5)．Type-2ceilscancreateheterogeneoussubcloneoffspring，buttype-lceilscannotgenerateoffspringcells． In comparison with type—lcells，type-2had strongerproliferation ability，and contained moreGl【／G1cells．Conclusion Carcinoma ceilsareheterOgene0usand type2 ceilspossesssomecharacteristicsoftumorstem cells． [KeywordslLaryngealneoplasm；Heterogeneity；Cancerstem cel1 ． 肿瘤干细胞 (CancerStem Cells)理论的建立，突破了传统 1．2．3 MTT法检测1型和2