VEGF反义寡核苷酸抑制胆囊癌生长增殖与凋亡的体外实验研究.docVIP

VEGF反义寡核苷酸抑制胆囊癌生长增殖和凋亡的体外实验研究 　　作者：李海军, 毛拉艾沙·买买提， 庞作良 作者单位：(新疆医科大学附属肿瘤医院肝胆胰外科, 新疆 乌鲁木齐 830011) 　　【摘要】 目的：探讨VEGF 反义寡核苷酸转染对人胆囊癌GBC-SD细胞生长、增殖和凋亡的影响。方法：运用Oligofectamine介导VEGF 反义寡核苷酸(antisense oligodeoxynucleotides，ASODN)和错义寡核苷酸(Scrambled Oligodeoxynucleotide，SODN)转染人胆囊癌(GBC-SD)细胞。采用MTT法测定转染后各组细胞的生长曲线及抑制率，流式细胞术检测转染后不同时间各组细胞凋亡情况。结果：SODN组、SODN十Oligofectamine组对GBC-SD细胞生长无显著影响(P0.05)，ASODN组及ASODN十Oligofectamine组则能显著抑制GBC-SD细胞生长(P0.05)，且ASODN十Oligofectamine组对GBC-SD细胞生长增殖抑制作用较ASODN组更为强(P0.05)。流式细胞术检测结果发现ASODN十Oligofectamine组能促进GBC-SD细胞凋亡(P0.05)。结论：VEGF ASODN转染能抑制胆囊癌GBC-SD细胞生长和增殖，Oligofectamine介导能明显增强VEGF ASODN的抑制作用;Oligofectamine介导VEGF 反义寡核苷酸转染能促进胆囊癌GBC-SD细胞的凋亡。 　　【关键词】 胆囊癌细胞株GBC-SD; VEGF; 反义寡核苷酸; Oligifectamine; 凋亡 　　The effect of oligofectamine mediated VEGF ASODN transfection onGBC-SD cell growth, proliferation and apoptosis in vitro 　　LI Hai-jun, Maolaaisha·Maimaiti, PANG Zuo-liang 　　(Department of Hepatobiliopancreatic Surgery, Affilicated Tumor Hospital, 　　Xinjiang Medical University, Urumqi 830011, China) 　　Abstract: Objective: To investigate the effect of oligofectamine mediated VEGF ASODN transfection on GBC-SD cell growth, proliferation and apoptosis in vitro. Methods: GBC-SD cells were transfected VEGF ASODN and SODN mediated by oligofectamine. The transfected GBC-SD cells of each groups were assessed the change of growth and proliferation activity by MTT, and apoptosis by flow cytometry. Results: The transfected GBC-SD cells growth and proliferation activity tested by MTT in groups of SODN and SODN+oligofectamine had not any difference as compared with control group (P0.05), but as for the cells in groups of ASODN and ASODN+oligofectamine, the growth and proliferation activity were significantly inhibited (P0.05), moreover, the effect of ASODN+oligofectamine was more significant than that of ASODN (P0.05). The results of flow cytometry revealed ASODN+oligofectamine could accelerate transfected GBC-SD cells apoptosis (P0.05). Conclusion: