黄淮海地区夏玉米纹枯病菌的融合群鉴定.doc

黄淮海地区夏玉米纹枯病菌的融合群鉴定 夏海波 伍恩宇 于金凤* 山东农业大学植物病理学系 泰安 271018( 摘 要：从黄淮海地区采集玉米纹枯病标样250余份，分离得到176个丝核菌菌株。融合群测定及5.8S rDNA-ITS区序列分析结果表明，这些菌株分别属于多核丝核菌的AG1-IA、AG1-IB、AG4-HG-I、AG-5、WAG-Z融合群及双核丝核菌的AG-A、AG-Ba融合群。其中AG1-IA是优势融合群，占分离菌株总数的64.20%，其次是AG-Ba，占12.50%，再依次分别是WAG-Z（10.23%）、AGI-IB（5.11%）、AG-4-HG-I（3.98）、AG-5（2.27%）和AG-A（1.70%）。其中AG-Ba融合群是国内首次在玉米上分离得到。从各融合群中选取代表性的菌株进行5.8S rDNA-ITS区序列分析结果表明，隶属不同融合群或亚群的菌株其5.8S rDNA-ITS区序列存在较大的差异，而相同融合群（或亚群）不同菌株之间其序列的一致性可高达97-100%。 5.8S rDNA-ITS区序列分析 The anastomosis groups of the corn sheath blight pathogen Rhizoctonia spp. in Huanghuai Plain and Haihe Plain of China XIA Hai-Bo WU En-Yu YU Jin-Feng* Department of Plant Pathology, Shandong Agricultural University, Tai’an 271018, China Abstract: One hundred and seventy-six isolates were obtained from corn sheath blight samples in Huanghuai Plain and Haihe Plain (including Shandong, Henan, Hebei Provinces and Northern regions of Jiangsu Province) of China. Anastomosis group identification and 5.8S rDNA-internal transcribed spacer (ITS) sequence analysis showed that the isolates belonged to multinucleate Rhizoctonia AG1-IA, AG1-IB, AG4-HG-I, AG-5 and WAG-Z and binucleate Rhizoctonia AG-A and AG-Ba. Of these, AG-1-IA was the major anastomosis group(AG)（64.20% of total isolates), followed by AG-Ba (12.50%), WAG-Z (10.23%), AG1-IB (5.11%), AG4-HG-I (3.98%), AG-5 (2.27%) and AG-A (1.70%). AG-Ba was isolated for the first time from maize in China. 5.8S rDNA-ITS sequence analysis showed that the isolates could be distinctly separated based on their AG types. The isolates belonging to the same AG (or sub-AG) showed 97%-100% sequence identity. Key words: Rhizoctonia solani, anastomosis group, 5.8S rDNA-ITS sequence 随着紧凑型玉米品种的大面积种植，玉米栽培密度提高，氮肥用量增加，形成有利于玉米纹枯病发生的小气候条件。自80年代以来，该病在一些地区为害日益严重，成为玉米上的一种重要病害(梁继农等 1997；唐朝荣等 2000)。我国不同玉米种植区引起玉米纹枯病的丝核菌的融合群不同（高卫东 1987；唐朝荣等 2000；李华荣和兰景华 1997；肖炎农等 2002）。但引起典型症状的主要是立枯丝核菌Rhizoctonia solani AG1-IA群。黄淮海地区是我国夏玉米主产区，玉米纹枯病对该地区玉米的危害呈逐年加重的趋势，目前国内尚无人对该地区玉米纹枯病菌的融合群类群进行系统研究。本