杜仲中木脂素类化合物纯化工艺研究.pdfVIP

302 2006 4 http:www hxtb org * # 柳娜 陈晓青 杜晖 薛小娟 欧阳冬生 ( 4 10083; # 4 10078) ( PDG) ( SDG) , , , 1 , UV IRMS H NMR , 9086% 917 3% , 4214% 4717% , Separation and Purification of the Liganas from EucommiaUlmoide Oliver * # Liu N a, Chen Xiaoqing , Du Hui, Xue Xiaojuan, Ouyang Dongsheng ( College of Chemistry and Chemical Engineering , Central South University, Changsha 4 10083; # Institute of Clinical Pharmacology, Central South University, Changsha 410078) Abstract A method f or separation and purif ication of the lignans pinoresinol diglu coside and syringaresinol diglucoside in Eucommia Ulmoide Oliver w ith solvent extractionmacroporous adsorption resinsilica gel column chrom atography w as established Solvent extraction w as choosen as the f irst purif ication Then , the purif ication of S8 macroporous adsorption resin w as studied The silica gel column chromatography was used to obtain tw o monomers of p inoresinol diglu coside and syringaresinol diglucoside The purity of the product w as deternm ined by RPHPLC detection The tw o crystals w ere identif ied as PDG and SDG by UV, IR, MS and 1H NMR Their purity w ere 9086 % and 9173% , and their recovery w ere 4214% and 4717% respectively The high purity monom ers of PDG and SDG can be obtained by this method, w hich is simple and of low cost Key