地方品种HR土鸡不同亚型禽白血病病毒共感染.pdfVIP

第二届全国禽病分子生物技术青年学者论坛论文集 地方品种HR土鸡不同亚型禽白血病病毒的共感染 赵成棣1，王波1，王健1，刘绍琼1，张培培1，孟凡娜1，张永光2，孙淑红埔 1山东农业大学动物医学院，泰安271018：2中国农业科学院兰州兽医研究所，兰州730046 摘要：本研究对HR土鸡中存在不同亚型禽白血病病毒 ALV 的共感染进行了探讨。分别采 同源性为99．0％。本研究显示，地方品种HR土鸡存在广泛的不同亚型ALV共感染，同时ALV-A和 ALV．J共感染同一个体的现象已经存在。 关键词：HR土鸡；J亚型禽白血病病毒；A亚型禽白血病病毒；共感染 in EvaluationonCo-infectionStatusofDifferent ofAvianLeukosisVirus Subgroups HRLocal Chicken Layer ZHAO B01，WANGJianl，LIU Pei．peil，MENGFan．ha, Cheng-dil，WANG Shao-qiong。，ZHANG ZHANG Yong-guan92，SUNShu-hong 1 of Medicineof 271 018，China；2 CollegeVeterinary ShandongAgdculturalUniversity,Tai’an Lanzhou Research Veterinary Institute，CAAS，Lanzhou，730046 Abstract：The evaluatestheco-infectionofavianleukosis HRIocal presentstudy virus ALV in flock．46cloacalswabsfromHR and455 whiteswereobtainedforthe layers paternalparent egg measurementof 30 wereusedfordetectionofALV-ABandALV-J p27antigen，whileeggyolk antibody ELISA．Theresultsshowedthatthe rateofcloacalswabsand whiteswere by positive egg 87％ 40／46 and andALV-J in were 74．7％ 340／455 ，respectively．ALV-ABantibodyeggyolk 0*／, 0／30 and local andALV-JwereallidemifiedIFAintheCEFcultureof5HR 80％ 24／30 ，respectively．ALV-A by paternalwhich testwere ALV-A parent p27antigen 20lOGNCl0924 III尔曲申．人．硕士研究z卜，E-mail：wb8532@163．corn。-人j℃为同等贡献作者 第二届全国禽病分子生物技术青年学者论坛论文集 HR332A，l identifiedwithtwo of towardALV-AandALv．JPCRfromthe 127bp were pairsprimers by strainHR332．Theresultof variation of ALv-Jindicatedthat analysisgp85 autogenous gene 924bp to the HR332Jand11 athomeand was between referencestrains abroad92．4*／,-97．9％．HR332A homology andreferencestrains Was90．1％、89．7％．andhasthe to RSA—A、MQNCSU highesthomology SDAU09E2athome．TheshowedthatthereWas co-infectionofdifferent ofALV study widely subgroups inHRloeal atthesame ofALV-AandALVr-J layers time．Besides．co-infection Wasfoundinthesame individual． local words：HR vianleukosisvirus leukosisvirus Key layerchicken；A subgroupJ；Avian subgroup A：Co-infection 禽自血病病毒 ALV 是以引起禽各种造血细胞肿瘤性增生为特征的一类反转录病毒群【