0.5哈蟆油匀浆对NK细胞活性和细胞免疫功能影响实验研究许洪亮,张贵君,渠磊,等.pdfVIP

第 1 6 卷 第 1 1 期 辽宁中医药大学学报 Vol. 16 No. 11 2014 年 11 月 JOURNAL OF LIAONING UNIVERSITY OF TCM Nov .，2014 DOI：10.13194/j.issn.1673-842x.2014.11.011 0.5%哈蟆油匀浆对NK细胞活性和细胞免疫功能影响实验研究 许洪亮，张贵君，渠磊，武亚楠，郑婧，郭怡祯，高晓美，蔡悦萍 （北京中医药大学，北京 100102 ） 摘  要：目的：观察0.5%（g/mL）哈蟆油匀浆对NK细胞活性和增强细胞免疫作用的影响。方法：用乳酸脱氢 酶LDH测定法研究对NK细胞活性的影响；用小鼠迟发型变态反应（DTH）研究对细胞免疫功能的影响。结果：经 口给予小鼠不同剂量的0.5%哈蟆油匀浆30 d，对小鼠NK细胞活性无影响；能增强小鼠迟发型变态反应，即能增强 细胞免疫功能。结论：0.5%哈蟆油匀浆对NK细胞活性无影响，根据动物实验结果，成人每日服用20 mL具有增强 细胞免疫的作用。 关键词：0.5%哈蟆油匀浆；免疫反应；迟发型变态反应；资源 ( ) 中图分类号：R285 文献标志码：A 文章编号：1673-842X 2014 11- 0033- 03 Study of NK Cell Activity and Cell-Mediated Immunity Effect on 0.5% of Oviductus Ranae Homogenate XU Hongliang，ZHANG Guijun，QU Lei，WU Yanan，ZHENG Jing， GUO Yizhen，GAO Xiaomei，CAI Yueping （Beijing University of Chinese Medicine，Beijing 100102，China） Abstract：Objective ：To study NK cell activity and cell-mediated immunity effect of 0.5%（g/mL） of Oviductus Ranae homogenate. Methods ：With LDH measurement，we studied the effect of 0.5% of Oviductus Ranae homogenate on mice NK cell activity. With DTH，we studied the effect of 0.5% g/mL of Oviductus Ranae homogenate on mice cell-mediated immunity. Results ：Mice took different doses of 0.5% of Oviductus Ranae homogenate after 30 days，and there was no influence on NK cell activity of Oviductus Ranae. It can enhance mice delayed allergy. In other words，it can strengthen cell-mediated immunity in mice. Conclusion ：0.5% of Oviductus Ranae homogenate has no influence on NK cell act