大鼠严重胸部创伤肺泡与间质巨噬细胞分泌TNF.docVIP

大鼠严重胸部创伤肺泡与间质巨噬细胞分泌TNF 　　作者：张伟，蒋耀光，李磊，谢志坚，吕凤林，胡承香　　(第三军医大学大坪医院全军胸心外科中心，重庆 400042;　　第三军医大学大学大坪医院野战外科研究所一室，重庆 400042) 　　摘要： 目的 观察大鼠严重胸部创伤后肺泡(AM)及间质(IM)巨噬细胞两类亚群分泌TNFΑ、IL6的差异，并探讨其意义。方法 利用小型多功能生物撞击机，以400kPa驱动压力对大鼠右侧上胸壁进行致伤，建立大鼠严重胸部创伤模型，支气管肺泡灌洗，机械结合酶消化法分离、培养肺泡及间质巨噬细胞，动态检测创伤前、创伤后2、4、8、16、24小时以及复合LPS攻击后肺泡及间质巨噬细胞分泌TNFΑ、IL6的水平。结果 创伤复合LPS攻击后，AM、IM分泌TNFΑ、IL6增加，伤后4、8、16小时时相点AM分泌TNFΑ显著高于IM; 伤后每个时相点IM分泌IL6均显著高于AM。结论 大鼠严重胸部创伤对AM、IM分泌TNFΑ、IL6具有不同影响，其在创伤后机体免疫功能紊乱的过程中具有不同作用，本研究为创伤性急性肺损伤的发病机制提供了一定的实验及理论依据。 　　关键词： 胸部损伤; 肺泡巨噬细胞; 间质巨噬细胞; TNFΑ; IL6; 大鼠 　　Effect of severe thoracic trauma combined with endotoxin infection on secretory function of alveolar macrophages and pulmonary interstitial macrophages in rats 　　ZHANG Wei,JIANG Yaoguang,LI lei,et al. 　　(Thoracic and Cardiovascular Surgery Research Center,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China) 　　Abstract： Objective To observe the different changes of alveolar macrophages and interstitial macrophages on secretory function. Methods Rat models of severe thoracic trauma were established,alveolar macrophages were collected from bronchoalveolar lavage fluid,mechanic flush and enzymatic digestion,and then separated and cultured. Secretory function of AM and IM were observed dynamically before trauma and 2,4,8,16,24h after trauma and challenged by LPS. Results A stable and reliable severe thoracic trauma model was successfully established with 400kPastrike on the upright chest of rat by a multiplefunction strike apparatus. Severe thoracic trauma and LPS challenged increased TNFΑ、IL6 secretion both in AM and IM. AM secreted more TNFΑ than IM at 4,8,16h after trauma,and IM secreted more IL6 than AM at every time point after trauma. Conclusion There effect of severe thoracic trauma on secretion of TNFΑ、IL6 between AM and IM is different. The present study provides valuable laboratorial and theoretical evidence for the research on ALI complicated with severe thor