肾上腺素对离体豚鼠左心室流出道组织自律性电活动的影响.docVIP

肾上腺素对离体豚鼠左心室流出道组织自律性电活动的影响 　　作者：陈立锋 徐涛 范凌 陈少峰 孙琳　 尹万斌　赵兰平 作者单位：(1.河北北方学院医学院生理教研室，河北 张家口 075000;2.河北北方学院附属第一医院心血管内科，河北 张家口 075000;3. 河北北方学院附属第一医院血液科，河北 张家口 075000;4.河北北方学院医学院临床医学本科2007级2班,河北 张家口 075000;5.河北北方学院医学院解剖教研室，河北 张家口 075000;6.河北北方学院医学院临床医学本科2006级2班，河北 张家口 075000) 　　【摘要】 目的：观察肾上腺素(E)对离体豚鼠左心室流出道组织自发慢反应电位的影响。方法：采用标准玻璃微电极细胞内记录技术，观察E对离体豚鼠左心室流出道组织自发慢反应电位的影响。观测指标有：最大舒张电位(MDP)，动作电位幅度(APA)，0期最大除极速度(Vmax)，4期自动除极速度(VDD)，自发放电频率(RPF)，复极50%和90%时间(APD50、APD90)。结果：用100Μmol/L E灌流，豚鼠左心室流出道自律组织动作电位APA明显增大(P0.01)，Vmax明显增快(P0.05)，MDP显著增大(P0.05)，APD90显著缩短(P0.01);RPF明显增快(P0.01)，VDD明显加快(P0.05)。结论：E对左心室流出道组织自律细胞有兴奋作用。 　　【关键词】 肾上腺素;电生理学技术/心脏;豚鼠;左心室流出通;自律细胞 　　As is a clinical cardiac stimulant,the epinephrine(E)has very important function in the cardiovascular system,but the effects on ventricular outflow tract cells havent been deeply reseached. The ventricular outflow tract used to be thought a hemal outflow path.With the development of clinical electrophysiology examination and radio frequency ablation technique in recent years,it was discovered that some arhythmias had relation with the abnormality of structure,appearance and function of ventricular outflow tract. By using standard intracellular microelectrode technique to record Aps,epinephrine was used to investigate the electrophysiological features of left ventricular outflow tract pacemaker cells of Guineapig.The purpose of our study was to observe the electrophysiological effects of epinephrine on pacemaker cells in left ventricular outflow tract,elucidate the mechanism(s)involved,provide electrophysiologic proof for the nosogenesis of some arrhythmias that originated from ventricular outflow tract. 　　1 MATERIALS AND METHODS 　　1.1 Specimen Preparation 　　Guineapig of either sex(weighing 200～350g,provided by Experiment Animal Research Center of Chinese Academy of Medical Sciences). Guineapigs were killed with a single blow on the head and the hearts were quickly removed and placed in warm(36.0±0.5)