血脂康预处理对心肌缺血再灌注大鼠炎症因子影响.docVIP

血脂康预处理对心肌缺血再灌注大鼠炎症因子影响 　　作者：聂毛晓,张雪娟,滕蕾 作者单位：(青岛大学医学院附属医院心内科，山东 青岛 266003) 　　【摘要】 目的 观察血脂康预处理对急性心肌缺血再灌注损伤大鼠炎症因子的影响，探讨其可能的作用机制。方法 健康SD大鼠32只，随机分为空白组、假手术组、缺血再灌注组和血脂康预处理组，各8只。血脂康预处理组每天灌胃给予血脂康胶囊0.09 g/kg，其余各组给予等量生理盐水，连续7 d。缺血再灌注组和血脂康预处理组大鼠均开胸并结扎冠状动脉前降支30 min，然后再通60 min;假手术组只开胸不结扎冠状动脉，空白组不做任何处理。采集大鼠腹主动脉血，分离血清，检测白细胞介素(IL)1Β、IL4、IL8、肿瘤坏死因子(TNFΑ)、肌酸激酶同工酶(CKMB)、肌钙蛋白I (cTNI)含量，并处死动物取出心脏，计算心肌梗死面积。结果 缺血再灌注组IL1Β、IL4、IL8、TNFΑ、CKMB和cTNI血清含量均明显高于空白组和假手术组(F=48.72～351.07,q=0.25～41.65,P0.01);而血脂康预处理组上述指标除IL4外均低于缺血再灌注损伤组(q=0.35～32.65,P0.05)，且血脂康预处理组大鼠心肌梗死面积小于缺血再灌注损伤组(F=231.26,q=17.21,P0.05)。结论 血脂康能抑制促炎因子IL1Β、IL8、TNFΑ的生成，并增加抗炎因子IL4生成，减轻心肌缺血再灌注损伤，对缺血再灌注损伤具有明显的保护作用。 　　【关键词】 心肌;再灌注损伤;炎症;白细胞介素类;大鼠,SpragueDawley 　　EFFECT OF XUEZHIKANG PRETREATMENT ON INFLAMMATORY FACTOR OF MYOCARDIAL ISCHEMIAREPERFUSION INJURY IN RATS NIE MAOXIAO, ZHANG XUEJUAN, TENG LEI (Department of Cardiology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China); [ABSTRACT] Objective To observe the effects of Xuezhikang capsules on inflammatory factor of myocardial ischemiareperfusion injury (MIRI) in rats and explore its possible mechanism of action. Methods Thirtytwo SD rats were evenly randomized to four groups: blank group, shamoperation group, MIRI group and Xuezhikang pretreatment group. The rats in Xuezhikang group were treated with Xuezhikang (0.09 g·kg-1·d-1, gastric gavage) and other groups were treated with the same amount of saline, for 7 days. Those in MIRI and Xuezhikang pretreatment groups underwent a thoracotomy and the left anterior descending branch of coronary artery was ligated for 30 minutes and then recanalized for 60 minutes; those in shamoperation group received only thoracotomy with no ligation of coronary artery; no any management was done for those in the blank group. Blood samples were extracted from the abdominal aorta, and the serum separated. The interleukin1Β (IL1Β), IL4, IL8, tumor necrosis factor alpha (TNFΑ), creati