MTT法测定卵巢癌患者单药与联合用药的体外敏感性.pdfVIP

·临床药学 · MTT 法测定卵巢癌患者单药与联合用药的体外敏感性 * ( ) 曹丽芝 ,任华益 ,刘人树 , 曾东向 湖南省肿瘤医院临床药学室 ,长沙市 410006 中图分类号 R 979 1 文献标识码 A 文章编号 1001- 0408 (2006) 15 - 1155 - 03 ( ) 摘 要 目的:采用四甲基偶氮唑盐 M T T 法测定卵巢癌患者术后单药与联合用药体外敏感性 ,为临床个体化化疗方案的选择提 供参考。方法 :采用临床常用的 20 种化疗方案所用药物对 18 例卵巢癌患者的新鲜标本进行 M T T 法体外敏感性测定。结果 :不同 类型的卵巢癌及同一类型的不同个体对化疗药物的敏感性差异较大;联合用药较单一用药的平均抑制率高。结论:卵巢癌化疗敏感 性个体差异大。M T T 法是一种简便而快速的测定肿瘤体外药物敏感性的试验方法 ,可用于卵巢癌临床个体化化疗方案的选择。 关键词 卵巢肿瘤 ; M T T 法 ;药物敏感性 ;化疗方案 Detection the Chemosensitivit y of Patients with Ovarian Cancer to Anticancer Drugs Used Either Alone or in Combination by MTT Assa y in Vitro CAO Lizhi ,R EN Hua yi ,L IU Renshu ,ZEN G Don gxiang (Dep t of Pharmacy , Hu’ nan Provincial Tumor Hos p ital ,Changsha 410006 ,China) ABSTRACT OBJ EC T IV E : To determine t he sensitivit y of p atient s wit h ovarian cancer to anticancer dru gs used eit her alone or combination after op eration in vit ro using M T T assay for references in t he selection of clinical individualized chemot herapy M E THODS :Chemosensitivit y of 18 cases of ovarian cancer f resh sp ecimen to 20 drug t herap ies commonly used in t he clinic were detected in vit ro by M T T assay R ESUL TS : The sensitivit y to anticancer a gent s varied greatly between different t yp es of ovarian cancer and between different individuals who share t he same t yp e of ovarian cancer The avera ge inhibition ratio on ovarian cancer cells was