不同提取方法对断肠草总生物碱提取率的影响.pdfVIP

第46卷第3期 农药 Vol. 46, No. 3 2007年3月 AGROCHEMICALS Mar . 2007 科技与开发- 不同提取方法对断肠草总生物碱提取率的影响 陈志慧，周家容 (仲恺农业技术学院化学与化工系，广州 510225) 摘要：采用索氏回流、常规浸提、超声波3种方法提取断肠草总碱，考察粗提物的提取率。结果粗提物的提取 率依次为1.22%、0.42%、1.57%。用超声波提取法比较了以95%乙醇、氯仿、甲醇为溶剂的提取率，结果依次 为1.21%、1.57%、1.54%。通过正交实验，得到超声提取优化工艺条件：70℃条件下，超声提取30 min×3次。表 明以氯仿为溶剂，用超声波提取法提取断肠草总碱，提取率最高。 关键词：断肠草；总生物碱；超声波；回流提取；浸提 中图分类号：TQ453.3文献标志码：A 文章编号：1006-0413(2007)03-0176-02 Effect of Different Methods on Extracting Ratios of Total Alkaloid of Gelsemium elegans Benth CHEN Zhi-hui, ZHOU Jia-rong (Department of Chemistry and Chemical Engineering , Zhongkai University of Agriculture Technology, Guangzhou 510225, China) Abstract: Total alkaloid of Gelsemium Elegans Benth extraction was conducted by way of circumfluence distillation, conventional extraction, and ultrasonic methods to investigate the extraction yields of crude extract . The results were 1.22%, 0.42%, 1.57%. Under ultrasonic extraction, comparing the ratio of total alkaloid with 95% ethanal, hloroform, methanol. The results were 1.21%, 1.57%, 1.54%. The optimum conditions for ultrasonic cxtraction were temperature 70 ℃, time duration 30 min ×3. The result indicated that ultrasonic was optimal extraction method. The ratio of total alkaloid in hloroform is higher than that in 95% ethanal, methanol. Key words: Gelsemium elegans Benth; total alkaloid; ultrasonic; circumfluence distillation; conventional extraction 断肠草(Gelsemium elegansBenth)，中草药名钩吻， 分。 为了充分利用断肠草，本研究选取了索氏回流、常 又名毒根、大茶药、胡蔓藤、野葛等 ，为马钱科 规浸提、超声波3种方法进行比较试验，以总碱粗提物的 [1] (loganiaceae)胡蔓藤属的全草 ，是我国传统的药用植物， 提取率为工艺筛选的一个评价指标，旨在寻求一种快速、