不同破壁方法对细菌产SOD活性的影响.pdfVIP

Sc ience and T echno logy o f Food Industry SOD , , ( 河南科技学院生物工程系, 河南新乡 453003 : 通过比较四种不同破壁方法对细菌产 SOD 活性 响的研究, 得到一种最有效的细菌破壁方法, 应用于从细菌 中提取 SOD 的破壁处理实验结果表明: 四种破壁处理间均达到显著差异水平, 并且助融剂破壁处理后 SOD 活性明 显高于其他三种处理方法, 即助融剂破壁效果最好, 是最适合细菌提取 SOD 的破壁处理方法 : 细菌, 破壁方法, SOD, 多重比较 Inf luence of different m ethods of ce ll- w all broken on SOD act iv ity produced by bacter ia L I L an, ZHANG M ing- xia, YUAN Jin- hu i ( D epartm en t of B ioeng ineering, H enan Institution of Science and T echno logy, X inx iang 453003, Ch ina Abstrac:t O ne k ni d o f the m o s t e ffe c tvi e m e thod of ce ll- w a ll b ro ke n w a s fo und a nd use d to e x tra c t SO D from b ac te ria c om pa ring the in flue nc e of se ve ra l d iffe re n tm e thod s on SO D a c tiv ity 1 T he re su lts o f e xpe rmi e nt show e d tha t fo u r d iffe re n tm e thod s ha d rem a rka b le d iffe re nc e, b e wt e e n them, a nd the SO D a c tiv ity o f be ing tre a te d by c o nso lve nt w a s hig he r tha n tha t o f th re e o the r m e tho d s, tha t is to sa y, the c o nso lve nt trea tm e nt m e tho d w a s the b e s t o ne, w hic h w a s the m o s t su ita b le m e tho d fo r SO D e x trac tion from b a c te r ia 1 Key w ords: ba c te ria; c e l-l w a ll b ro ke n m e tho d; SO D; m u lt-i c om pa red : T S20113 : A : 1002- 0306( 2008 0 10- 0108- 04 ( Superox ide D ismutase, SOD 1 M ann K eilin 1838 111 , , M ccord 1969 ( SOD SOD ; T ris- HC l pH 812, 50mm o l/L; HC l 10mmo l/L; , SOD O-