咬合重建对大鼠三叉神经节P物质表达影响的研究.docVIP

咬合重建对大鼠三叉神经节P物质表达影响的研究* 摘要 目的:研究咬合重建对大鼠三叉神经节P物质(substance P, SP)表达的影响。方法:Wistar雄性大鼠30只,随机分为3个实验组及相应的正常对照组,每组5只。实验组动物间断磨除右上、下颌磨牙牙冠至龈下,有二组分别第3周、第9周停止磨牙，任其自行萌出，恢复咬合关系。双侧三叉神经节（trigeminal ganglia，TG）切片行SP免疫组织化学反应(SABC法)。光镜观察拍片,并用Image Pro Plus 5.1图像分析软件进行测定。结果与对照组对照。SPSS10.0软件行统计分析。结果:单侧咀嚼实验组咀嚼侧和非咀嚼侧TG 内SP免疫阳性神经元百分比与对照组比较显著降低(P0.01，P0.05), 其非咀嚼侧明显低于咀嚼侧(P0.01)。早期恢复咬合实验组TG内免疫阳性神经元百分比与对照组比较无差别(P0.05)，其咀嚼侧与非咀嚼侧比较无差别(P0.05)。晚期恢复咬合实验组TG内免疫阳性神经元百分比与对照组比较显著降低(P0.01，P0.05)，其非咀嚼侧明显低于咀嚼侧(P0.05)。结论:早期恢复咬合关系TG内SP表达可恢复正常，晚期恢复咬合关系SP表达不能恢复正常，SP参与了单侧咀嚼引起的颞颌关节病的病理变化过程 关键词 P物质;三叉神经节;咬合重建;大鼠 Expressions of Substance P in Trigeminal Ganglia in Rats with Occlusal Reconstruction [ Abstract] Objective:Our aims were to study the expressions of substance P(SP) in trigeminal ganglia（TG） in rats with occlusal reconstruction，and to discuss the mechanism of temporomandibular disorders.Methods:30 Wistar male rats were randomly divided into 3 experimental groups and 3 control groups，5 rats in each group .The molar of right maxillary and mandibular of rats in experimental groups were ground to the gingival level without occlusal contact. The occlusal contact was recovered by stoping grounding molar of the rats.The section of trigeminal ganglia underwent the immunohistological study to evaluate the expressions of SP by using SABC method.Light microscope and microscoic photo analytic software were employed to detect the percentage of SP positive neurons in frozen section of TG in 30 rats. SPSS10.0 statistical software were used for statistical analysis.Results: The percentage of SP positive neurons in TG with Unilateral Chew experimental group was significantly decreased compared with control group (P0.01，P0.05)，The decreased extent in non-chewing side was much lower than that in the chewing side(P0.05).There was no significantly difference in the percentage of SP positive neurons between the early occlusal reconstruction experiment group and control group(P0.05) and there was no signific