噻托溴铵干预对慢性阻塞性肺疾病大鼠气道重塑和MMP9表达的影响.pdfVIP

·103· 噻托溴铵干预对慢性阻塞性肺疾病大鼠 气道重塑和MMP一9表达的影响 李莉芳李建强 【摘要】 目的观察噻托溴铵早期干预对慢性阻塞性肺疾病(COPD)大鼠模型气道重塑及基质金属蛋 干预组(C组)，采用熏烟并气管内注入脂多糖的方法建立COPD模型，c组于早期给予噻托溴铵雾化干预。观察 肺组织病理形态学变化。图像分析测量小气道管壁和平滑肌厚度，采用免疫组织化学法测MMP-9蛋白。结果 与B组相比，C组大鼠肺组织气道重塑病理变化减轻，MMP一9降低。结论噻托溴铵对COPD气道重塑有抑制 作用，可降低MMP-9表达。 【关键词l肺疾病，慢性阻塞性；明胶酶B；气道重塑；噻托溴铵 Effectsof bromideon andMMP-9 inchronicobstructive tiotropium airwayremodeling expression pulmonary d妇ratmodelLI Second Medicine， Affiliated of Li-fartg,L1Jian-qiang．Department矿RespiratoryHospital MeScal Shanxi Unweary,Taiyuan030001，China Toobservetheeffectsof bromideOil andthe of 【Abstract】Objective tiotropium airwayremodelingexpression mrs．Methods three matrix COPD Wistarratswererandomizedinto metalloproteinase-9(MMP-9)in groups：normal control bromidetreated COPDratmodelwag group(groupA)，COPDgroup(groupB)，tiotropium group(groupC)．The constructedintratrachealinstillationof twiceand to C lipopolysaccharideexposurecigarette by daily smoking．Group inhaled bromide from 8．The ofMMP-9in and tissue received bronchi tiotropiumdailystartingday expression lung wasdetectedimmunohistoehemical administrationof bromideresultedinstatis- Daily by analysis．Results tiotropium withthe