5个湖泊河川沙塘鳢(Odontobutis+potamophila)种群线粒体细胞色素b基因的遗传变异分析.pdfVIP

，Lake Sci．(湖泊科学)，2015，27(4)：693—699 DOI10．18307／2015．0418 ⑥2015 LakeSciences Journal by of 5个湖泊河川沙塘鳢(Odontobutis 色素b基因的遗传变异分析8 徐宇1，钟立强1’2，李潇轩1，朱锡和‘，王崇华1，史杨白H+ (1：江苏省淡水水产研究所，南京210017) (2：农业部淡水渔业健康养殖重点实验室，浙江省淡水水产研究所，湖州313001) 摘要：通过对5个湖泊的河川沙塘鳢种群的线粒体DNA细胞色素b基因进行PCR扩增、测序，获得1141bp的序列全 长．序列分析显示，cyt 性．太湖种群与大纵湖种群问的遗传距离最近，为0．137，巢湖种群和大纵湖种群之间遗传距离最远，为0．424．分子方差 b基因序列构建的UPGMA系统进化树显示，5 分析表明，群体间遗传分化系数F。为O．531，变异来自群体内及群体间．eyt 个种群分化成不同的分支系谱，种群间存在的基因交流较少． 关键词：河川沙塘鳢；线粒体DNA；细胞色素b基因；遗传变异 Genetic ofOdontobutis fromfivelakesbasedonMitochon- variation potamophila analysis drial DNAb cyt XU Yul，ZHONG Xiaoxuanl，ZHUXihel，WANG Yangbail Liqian91一，LI ChonghualSill FisheriesResearchInstitute (1：Freshwater ofJiangsuProvince，Nanjing210017，P．R．China) FishHealthandNutrition InstituteFreshwater Laboratory of of (2：Key of Zh巧iangProvince，Zh巧iang 313001，P．R．China) mtDNAb ofOdontobutis fromfivelakeswas and showedthatthe Abstract：Thecyt gene potamophila amplifiedsequenced．Result content alittle that the ofll41 varia- average ofA+T(55．8％)washigherthanofG+c(44．2％)，inalignedsequencesbp．806 blesitesweredetectedand87 wererecovered．The andnueleotidewere0．9694-0．012 diversity diversity haplotypes haplotypes distance Taihu andLake dis— and betweenLake 0．20081±0．00742，respectively．Thegenetic population Dazonghupopulation the to thatbetweenLakeChaohu andLake wasthe to playedlowest，up0．137，while populat