湖光岩玛珥湖春季浮游植物对溶解态氮的吸收-.pdfVIP

J.Lake Sci.(湖泊科学),2015,27(3):527-534 http:// .E-mail:jlakes@ ©2015 by Journal of Lake Sciences 湖光岩玛珥湖春季浮游植物对溶解态氮的吸收∗ 1 1∗∗ 1 2 张国维 ,李长玲 ,黄翔鹄 ,古滨河 (1:广东海洋大学水产学院,湛江524088) (2:美国佛罗里达大学土壤和水科学系,佛罗里达州32611) 15 摘　 要:利用 N稳定同位素示踪技术,采用现场挂瓶培养的方法测定了湖光岩玛珥湖浮游植物群落对铵态氮、硝态氮和 尿素态氮的吸收速率,研究了湖光岩玛珥湖浮游植物群落氮吸收及其吸收动力学特征.结果表明:湖光岩玛珥湖共检测 到浮游植物7 门54种(包括变种和变型),主要为蓝藻门、硅藻门和绿藻门种类,分别占浮游植物总量的44.68% 、 26.70% 和19.21% ,其中水华微囊藻(Microcystisflos-aquae)与铜绿微囊藻(Microcystis aeruginosa)为绝对优势种,优势度分 别为0.39与0.28.湖光岩玛珥湖浮游植物群落对铵态氮的绝对吸收速率最高,分别是对硝态氮、尿素态氮绝对吸收速率 的5.8和4.2倍,占3种溶解态氮总吸收量的73.3% .铵态氮、硝态氮和尿素态氮的相对优先指数分别为2.907、0.190 和 1.192,说明浮游植物群落优先吸收铵态氮,其次为尿素态氮,最后为硝态氮.铵态氮、硝态氮和尿素态氮的周转时间分别为 3.72、57.03和9.07h.湖光岩玛珥湖浮游植物对溶解态氮的吸收可用Michaelis-Menten酶动力学方程描述,最大比吸收速 率表现为铵态氮尿素态氮硝态氮,亲和力表现为硝态氮铵态氮尿素态氮.湖光岩玛珥湖浮游植物群落对铵态氮 具有较高的吸收潜力,并且对硝态氮具有一定的亲和力,具备利用硝态氮的能力. 关键词:湖光岩玛珥湖;浮游植物;稳定同位素;氮吸收;吸收动力学 Uptake of dissolved nitrogen by phytoplankton inspring in Huguangyan Maar Lake 1 1 1 2 ZHANG Guowei ,LI Changling ,HUANGXianghu GU Binhe (1:Fisheries College,Guangdong Ocean University,Zhanjiang 524088,P.R.China) (2:The Soil and WaterScienceDepartment,University of Florida,Florida32611,USA) 15 Abstract:Using N stable isotope tracer technique by adopting the in-situ flask culture,the uptake rates of ammonium,nitrate and urea by phytoplankton community were measured in Huguangyan Maar Lake,in order to characterize the nitrogen absorption and the characteristics of absorptionkineticsbyphytoplanktoncommunity in springinthelake. Theresultsshowedthattherewere 54 species (includingvarietiesandformsbelongingto7 phyla)ofphytoplanktoninthelake,mainlyfromtheCyanophyta,Bacilla- riophyta and Chlorophyta,which accounted for 44.68% ,26.70% and 19.21% ,respectively. Microcystisflos-aquae and M. aeruginosa were the absolutedominant species,andthedominanceindexeswere0.39 and0.28,respectively. Uptakerateof am- monium of p