芦笋性别决定与性别分化研究进展.pdfVIP

2010, 11( 5) : 600604 Journa l of P lant G enetic R esources 芦笋性别决定与性别分化研究进展 周劲松, 汤泳萍, 盛文涛, 罗绍春, 陈光宇 ( , 330200) : 从芦笋性别表现及其决定的遗传基础性别 化途径, 性别决定基因的定位以及性别 化特异表达基因的 离与 析等方面来综述芦笋性别决定与性别 化最新研究进展目前, 已构建了围绕芦笋性别决定基因M 比较精细的遗传图谱, 将M 定位在 L5 染色体着丝点附近的 0. 63 cM 区域内, 并构建了含有 8 个跨叠克隆群的物理图谱, 但由于大量重复序列的存 在, 跨叠克隆之间的空隙不能闭合 同时先后 离得到 11 个芦笋花器官发育特异表达基因, 并通过序列 析和原位杂交等技 术对这些基因的功能进行了 析最后, 对今后进一步研究提出了建议 : 芦笋 性别决定 性别 化 Progress in Research on Sex Determ ination and Differentiation in Asp aragus off icinalis L. ZHOU Jinsong, TANG Y ongp ing, SH EN G W entao, LUO Sh aochun, CH EN Gu angyu (B iotechnology Center, J iangx iA cademy of Agricultural Sciences, N anchang 330200) Ab stract: T he paper generally introdu ced the progress on sex determ ination and differentiation inA s aragus of f icinalis L. , including sex expression, genetic b asis of sex de term inat ion, the p athw ay of sex d ifferent iat ion, sex de term in ing gene m app ing, deve lopm ent of m arkers for the ident ification o f sex, and iso lation and funct ion analysis o f sex d ifferen tial expression gene. So far, a reg ion called theM locu s h ad located on a p air of hom om orph ic sex chro m osom es con tro ls the sexu al d mi orph ism in asparagu s and the reg ion h ad been delmi ited to 0. 63cM near the centro m ere reg ion s of L5 chrom osom e. A physica lm ap enclosing the sexdeterm in ing reg ions w ith eight Con tigs h ad been estab lished. H ow ever, the gaps betw een the contigs could not be closed du e to a plethora of rep etitive e lem ents. On the other h and, 11 floral organ deve lopm enta l expression genes had a lso been ch aractered and ana lyzed. F inally, sev eral suggestion s abou t the study of