小鼠受亚致死剂量60Coγ射线照射后胸腺细胞凋亡的研究.pdfVIP

980206.htm 中华放射医学与防护杂志 科技期刊 Chinese Journal of Radiological Medicine and Protection 1998年 第2期 No.2 1998 60 小鼠受亚致死剂量 Co γ射线照射后 胸腺细胞凋亡的研究 吴玮　孔宪涛　杨如俊　李莉　闵锐　张玲珍　施笑梅　周炳荣 60 　　目的　探讨小鼠受亚致死剂量 Co γ射线照射后胸腺细胞凋亡的变化规律，为研 60 究辐射后免疫功能的重建打下基础。方法　采用 Co γ射线，2.0，4.0，6.0 Gy单次全 身照射，用PI染色流式细胞仪分析，DNA琼脂糖凝胶电泳及细胞激活增殖能力的测定 等方法观察胸腺细胞凋亡的规律。结果　胸腺细胞照后2小时即出现明显的细胞凋亡， 4～8小时达高峰，后渐减少，4.0 Gy，6.0 Gy在36小时内的凋亡率均比2 Gy高，照后10 天三个剂量组的凋亡率基本接近正常；DNA琼脂糖凝胶电泳有大量的小分子片断，呈 典型的梯状图谱；4 Gy组用PHA、rIL-2不同组合刺激受照组细胞，发现胸腺细胞凋亡 60 率有不同程度的下降。结论　亚致死剂量 Co γ射线照射后小鼠胸腺细胞出现明显的 凋亡，4 Gy照射剂量可能是胸腺细胞达凋亡高峰的最适剂量，PHA、rIL-2联合对辐射 诱导的胸腺细胞的凋亡有明显的保护作用。 60 　　关键词　胸腺细胞　细胞凋亡　 Co γ射线　全身照射 Apoptosis of thymocytes in sublethally 60Co γ-irradiated mice.Wu Wei,Kong Xiantao, Yang Rujun,et al.Clinical Immunology Center of PLA,Shanghai Changzheng Hospital,The Second Military Medial University.Shanghai 200433,China. Objective The aim of this study was to investigate the apoptosis of thymocytes in 60Co γ- irradiated mice.Methods By flow cytometry,electrophoretic analysis of DNA and lymphocyte-stimulating test,we determined the changes of thymocyte apoptosis in mice.The mice were whole-body irradiated with 2.0,4.0 and 6.0 Gy 60Co γ-rays.Results While the thymocytes underwent apoptosis within 2 h after irradiation,fragmentation rate increased approximately 4～8 h after exposure to radiation doses of 2.0—6.0 Gy.Agarose gel electrophoresis of DNA in the 27 000×g supernatant of lysates obtained from thymocytes of irradiated mice showed ladder pattern.We found that PHA and rIL-2 prevented thymocytes from undergoing apoptosis induced by γ