用半定量RT_PCR法分析基因表达水平的影响因素.pdfVIP

2009,37(8): 28~ 30 Guizhou Agricultural Sciences [ ] 1001-3601( 2009) 08-0510-0028-03 用半定量RT-PCR 法分析基因表达水平的影响因素 1, 2 1 1 2 2* 陈名红 , 陈毅坚, 叶艳青 , 苏 源 , 李成云 ( 1. , 650031; 2. , 650201) [ ] 半定量逆转录聚合酶链式反应( Sem-i QRT-PCR) 以其灵敏简捷及特异性高等优点已逐渐成 分析基因表达 水平的一种有效手段着重对用半定量 RT-PCR 法分析基因表达水平的相关影响因素进行了综合论述, 并讨论了半定量 RT-PCR 法在基因表达研究方面的应用潜力 [] 半定量逆转录聚合酶链式反应; 基因表达; 影响因素 [] S188 [] A The Factors Affecting Analysis of Gene Expression Level by Sem-i QRT-PCR Techni ue 1,2 1 1 2 2* CHEN Ming-hong , CHEN Y-ijian , YE Yan-qing , SU Yuan , LI Cheng-yun ( 1. The School of Chemistry and Bio-technique of Yunnan Nationalities University , Kunming, Yunnan 650031 2. Key Laboratory of The Ministry of Educational f or Agro-biodiversity and Pests Control, Yunnan Agricultural University , Kunming, Yunnan 650201, China) Abstract: Sem-i QRT-PCR is a rapid, simple and highly specific means in analysis of gene expression level in recent years. This paper describes factors affecting analysis of gene expression level by the Sem-i QRT-PCR technique comprehensively, and also discusses the potential application of the Sem-i QRT-PCR technique in gene expression research. Key words: Semi-QRT-PCR; gene expression; influencing factor , 1 RNA , 1. 1 ( semi-quantita- RNA , tive reverse transcription and polymerase chain re- RNA, RNA action,Sem-i QRT-PCR) ,RNA [ 1-3] ; , [8] RNA cDNA, RNA cDNA cDNA,PCR , RNA RNA, mRNA RNA , [4-5]