表皮细胞生长因子在体诱导猪骨髓间充质干细胞向皮肤组织分化作用的研究.docVIP

表皮细胞生长因子在体诱导猪骨髓间充质干细胞向皮肤组织分化作用的研究 　　作者：都义日，付小兵，李存保，孙同柱，方利君(解放军总医院304临床部全军创伤修复重点实验室，北京 100037;内蒙古医学院基础部，呼和浩特 010059) 　　摘要： 目的 观察表皮细胞生长因子(EGF)在体内是否具有诱导骨髓间充质干细胞(MSCs)向皮肤组织分化的作用，及其在皮肤创面愈合中的作用。方法 抽取小型猪骨髓，体外分离、纯化和培养MSCs，并用5溴脱氧尿嘧啶(BrdU)进行标记。6只小型猪共48个创面，随机分为EGF+MSCs治疗组(A组)、MSCs治疗组(B组)、EGF治疗组(C组)和生理盐水对照组(D组)。观察伤后治疗3、7、14、21及84天的创面，并用病理学、免疫组化方法对创面的修复质量进行评估。结果 A组的皮肤创面愈合速度明显快于其它治疗组，并且在皮肤愈合的组织病理等级评分上优于B、C和D组。A组表皮层BrdU和CKp双染阳性细胞比B组更为多见。结论 EGF具有在体促进猪骨髓间充质干细胞向表皮细胞分化，加快皮肤创面愈合速度和提高愈合质量的作用。 　　关键词：表皮细胞生长因子;骨髓间充质干细胞;皮肤创面 　　In vivo studies on the differentiation of mesenchymal stem cells to skin tissues induced by epidermal growth factor 　　DU Yiri,FU Xiaobing,LI Cunbao,et al. 　　(Key Laboratory of Wound Repair,304 Medical Department,The General Hospital,PLA,Beijing 100037,China) 　　Abstract： Objective To investigate the role of epidermal growth factor (EGF) on the differentiation of mesenchymal stem cells to skin tissues in vivo and its effect on skin wound healing.Methods MSCs was isolated from porcine marrow,then purified and cultured with 5bromodeoxyuridine(5-BrdU) as a label in vitro.Fortyeight skin wounds on the 6 pigs were divided into EGF+MSCs (A),MSCs(B),EGF(C) and contrast (D) groups randomly and treated correspondingly.Wound closing index (WCI),cell morphology and histological changes in the defected regions were evaluated 3、7、14、21、84 days after treatment.Results Curative speed of defected regions of group A was faster than that of the other groups;Hierarchical scores of histopathology on the specimens of group A was also higher than that of the other groups.The dualpositive stained cells by BrdU and CKp were more in the specimen of the group A than that of group B.Conclusion EGF can induce MSCs to differentiate into epidermal cells,and promote the repair of skin wounds as well. 　　Key words：epidermal growth factor;marrow mesenchymal stem cells;skin wound 　　骨髓间充质干细胞(mesenchymal stem cells,MSCs)是一类具有多向分化潜能的干细胞，在不同诱导条件下可以向不同的组织分化。体外实验表明，经分离培养的MSCs在E