旋覆代赭汤对胃动力低下大鼠胃窦平滑肌细胞胃泌素受体及其管活性肠肽2受体mRNA表达的影响.pdfVIP

旋覆代赭汤对胃动力低下大鼠胃窦平滑肌细胞胃泌素受体及管活性肠肽2受体 mRNA表达的影响 税典奎 谢胜 （柳州市中医院，广西 柳州 545001） 摘要：目的 观察旋覆代赭汤对胃动力低下大鼠胃窦平滑肌细胞胃泌素受体（GASR ）及血 管活性肠肽 2 受体（VIPR2 ）mRNA 表达的影响。方法 将 70 只大鼠随机分为正常组和模型 组，正常组 10 只，模型组 60 只。模型组每只大鼠按 10g/kg 体重的甘草煎剂灌胃后，随机 分为模型3 天组、模型7 天组、旋覆代赭汤低、中、高剂量组及吗丁啉组。吗丁啉组予0.27mg/ml 的吗丁啉混悬液灌胃，旋覆代赭汤低、中、高剂量组分别予浓度为 0.369g/ml、0.738g/ml、 1.476g/ml 的旋覆代赭汤灌胃，给药 5 天。采用原位杂交法检测大鼠胃窦平滑肌细胞 GASR 及 VIPR2 的 mRNA 的表达情况。结果 一定剂量甘草煎剂可复制大鼠胃动力低下模型，旋 覆代赭汤可使胃动力低下大鼠胃窦平滑肌细胞 GASR mRNA 表达的阳性细胞平均光密度升 高，平均灰度值降低；而 VIPR2 mRNA 表达的阳性细胞的平均光密度降低，平均灰度值升 高，结果均有显著性差异（P0.05 或 P0.01 ）。结论 旋覆代赭汤可通过调节脑肠肽受体在 胃窦组织中的表达达到促胃动力的作用。 关键词：胃动力低下；胃泌素受体；血管活性肠肽2 受体；旋覆代赭汤 The effect of XFDZD on the mRNA expression of GASR and VIPR2 in low gastric motility rat gastric antral smooth muscle cells SHUI Dian-kui, XIE Sheng （Chinese medicine hospital of Liuzhou, Guangxi,Liuzhou 545001 ） [Abstract] Objectives: To investigate the effect of XuanFuDaiZhe Decoction (XFDZD) on the mRNA expressing of gastrin receptor(GASR) and vasoactive intestinal peptide receptor 2(VIPR2) in low gastric motility rat gastric antral smooth muscle cells. Methods: 70 Wistar rats were randomly divided into normal group (10 rats) and model group (60 rats). Each rat of model group was taken liquorice decoction(10g/kg) by gastric irrigation. After gastric irrigation , model group were divided into model group of 3 days, model group of 7 days, low, hit, high dosage groups of XFDZD, group of domperidone. Domperidone group were given domperidone (0.27mg/ml) by gastric irrigation, low, hit, high dosage groups of XFDZD were given XFDZD of different concentrations (0.369g/ml, 0.738g/ml, 1.476g/ml) by gastric irrigation. Administration for five days, ob