阴道上皮细胞的抗念珠菌作用及雌激素对此的影响.pdfVIP

现代妇产科进展 2004 年 11 月第 13 卷第 6 期 　Prog Obstet Gynecol ,Nov. 2004 ,Vol. 13 ,No. 6 ·论著· 阴道上皮细胞的抗念珠菌作用及雌激素对此的影响 牛战琴 ,石一复 , 吕时铭,叶 　红 (浙江大学医学院附属妇产科医院 ,杭州 　310006) 　　【摘要】　目的 :研究阴道上皮细胞对白色念珠菌生长的抑制作用及雌激素对此作用 的影响。方法 :用酶消化法分别分离雌激素处理组、动情间期组和去势组小鼠阴道上皮细 胞作为效应细胞 , 白色念珠菌芽生孢子作为靶细胞,将效靶细胞共同孵育 9h ,测定白色念 珠菌生长抑制率 ,观察其生长密度及透射电镜下超微结构的改变。结果 : 白色念珠菌与阴 道上皮细胞共同孵育后 ,生长受到抑制 ,生长密度降低 ,超微结构表现为与阴道上皮细胞 靠近的念珠菌细胞壁崩解 ,细胞膜连续性破坏 ,甚至细胞器肿胀溶解。雌激素处理组与去 ( ) 势组和动情间期组比较 ,阴道上皮细胞对念珠菌的生长抑制率降低 P 0. 05 。结论 :小 鼠阴道上皮细胞具有天然的抗白色念珠菌作用,雌激素能降低此抑制作用。 【关键词】　上皮细胞 ,阴道;念珠菌 , 白色;生长抑制 ;雌激素 ;小鼠 ( ) 中图分类号:R339. 2 　文献标识码 :A 　文章编号 :1004 - 7379 2004 06 - 0416 - 03 Anti Candida activity mediated by vaginal epithelial cells from mice and the effect of estrogen on it. Niu Zhanqin , Shi Yifu , L üShiming , et al . Women′s Hospital , School of Medicine , Zhejiang Uni versity , Hangzhou 310006 【Abstract 】　Objective :To investigate the growth inhibition to Candida albicans mediated by vaginal epithelial cells and determine if estrogen affected the activity. Methods :C57BL/ 6J mice vaginae from estrogen treatment group , diestrus group and ovariectomized group were excised respec tively. The vaginae were dissociated into single cell suspension by dispase and collagenase Ⅰ. Im munohistochemical analyse of the separated cells demonstrated that over 80 % were vaginal epithelial cells. The epithelial enriched cells were used as effector cells. Stationaryphase blastoconidia of C. albicans strains were used as target cells. After 9h coincubation of effector ( E) with target ( T) at various ratios ,