王不流行增乳活性单体对奶牛乳腺上皮细胞增殖及泌乳性能的影响.pdfVIP

66  《乳业科学与技术》 2010 年第2 期 （总第 141 期）    王不留行增乳活性单体对奶牛乳腺上皮细胞增殖及泌乳性能的影响 * 万中英，佟慧丽，李庆章，高学军 东北农业大学乳品科学教育部重点实验室，黑龙江哈尔滨 150030 摘 要：应用 CASY 细胞分析仪及 HPLC 分别检测王不留行邻苯二甲酸二丁酯对乳腺上皮细胞增殖、细 胞活力及乳腺上皮细胞分泌 β-酪蛋白、乳糖的影响，研究王不留行增乳活性单体邻苯二甲酸二丁酯对奶 牛泌乳中期乳腺上皮细胞增殖和泌乳性能的影响。试验结果表明，王不留行增乳活性单体成份邻苯二甲 酸二丁酯对奶牛乳腺上皮细胞的增殖和细胞活力提高均显著（P0.05 ）；能显著提高乳腺上皮细胞 β-酪 蛋白的表达（P0.05 ），也能提高乳糖的分泌（P0.05 ，P0.05 ）。因此，王不留行增乳活性单体邻苯二 甲酸二丁酯可显著提高乳腺上皮细胞的增殖和泌乳能力。 关键词：王不留行；邻苯二甲酸二丁酯；奶牛乳腺上皮细胞 中图分类号：R931.7 文献标识码：A 文章编号：1671-5187(2010)02-0066-03 Effects on the Proliferation and Lactation Ability of Dairy Cow Mammary Gland Epithelial Cells by Semen Vaccariae Active Isomer * Wan Zhongying, Tong Huili, Li Qingzhang, Gao Xuejun Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin of Heilongjiang 150030, China Abstract: The proliferations and viabilities of mammary gland epithelial cells were determined by CASY®-technology, whereas the expressions of β-casein and lactose of mammary gland epithelial cells were determined by HPLC. Dairy cow mammary gland epithelial cell was treated at different dibutyl phthalate (DBP) concentration, in order to investigate the influence of Semen Vaccariae active monomer on the proliferation and lactation of bovine mammary gland epithelial cells. The results showed that Semen Vaccariae active monomer significantly increased the proliferations and viabilities of mammary gland epithelial cells (P0.05). Furthermore (Dibutyl phthalate, DBP) could significantly enhanced the expression of β-c