n-3多不饱和脂肪酸对高脂饲养大鼠大血管的保护作用机制.pdfVIP

·898· n．3多不 饱和脂肪酸对高脂饲养大鼠大血管的保护作用机制 肖或君，母义明，潘长玉，陆菊明，柳红芳，詹晓蓉，陆祖谦 解放军总医院 内分泌科。北京100853 摘要：目的探讨n一3脂肪酸对高脂诱发的胰岛素抵抗大鼠的血管内皮细胞的保护机制。方法45只Wistar雄性大鼠随机 分为对照组、高脂饲养(HD组和HF+n一3脂肪酸干预组。饲养1l周后处死大鼠，测定血糖、胰岛素、血脂、体重以及内 B／IKBd 脏脂肪含量、主动脉组织形态学改变等，免疫组织化学法测定主动脉血管内皮细胞的ICAM一1、MCP一1和NF—K 脏脂肪占体重百分比均明显低于HF组，HDL—C则明显高于HF组，与对照组无明显差别；n一3脂肪酸组大鼠主动脉主动 脉内膜完整无内皮细胞脱落现象，内膜下泡沫细胞及炎细胞浸润减少，内膜的NF—KB呈弱阳性表达，IKBoc呈阳性表达， ICAM—l和MCP-I均呈阴性或弱阳性表达。结论n-3脂肪酸能有效阻断高脂饲养大鼠的胰岛素抵抗和大血管病变。 关键词：脂肪酸类，∞3；糖尿病血管病变；大鼠 中图分类号：R58 文献标识码：A 文章编号：1005一l139(2009)06—0898-03 Protectiveeffectsofn-3 acidsonmacrovascularinduced polyunsaturatedfatty disease byhigh fat．fedinrats X1AO Yu-jun，MU Hong-fang，ZHAN Yi-ming，PANChang-yu，LUJu-ming，LIU Xiao—rong，LUZu-qian of PLAGeneral 100853，China DepartmentEndocrinology，ChineseHospital，Beijing author：MU CorrespondingYi—ming．Email：muyiming@301hospital．corn．cn To the effectsofn-3 endothelialcell Abstract：Objectiveinvestigateprotective polyunsaturatedfattyacids(PUFA)Oildamage inducedfatininsulin—resistantrats．MethodsWistarillalerats 5-6weekswere dividedintocontrol high Forty-five by aged randomly group，high andn-3PUFA ratswelesacrificedafter11weeks．Levelsofblood fat-fed(I-W)groupgroup．The glucose，insulin， fatand of andNF-KB／IKB and in expressionICAM-l，MCP-l lipid。visceral we犯measured．Bodyweighthistologicalchange aortaweredetected．ResultsThe fatand wellasthe ofblood ration，as levels bodyweight，visceralbodyweight glucose，insulin，