al12 水稻苗期低温白化突变体al12的超微结构与基因定位.pdfVIP

遗 传 学 报 Acta Genetica Sinica , December 2006, 33 (12)：1112–1119 ISSN 0379-4172 Ultrastructure and Gene Mapping of the Albino Mutant al12 in Rice (Oryza sativa L.) XIA Jiu-Cheng1,2,3, WANG Yu-Ping1,2, MA Bing-Tian1,2, YIN Zhao-Qing1,2, HAO Ming1,2, KONG De-Wei1,2 , LI Shi-Gui1,2,① 1. Rice Institute of Sichuan Agricultural University, Wenjiang 611130, China; 2. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China; 3. Panzhihua University, Panzhihua 617000, China Abstract: Seedling albino mutation resistant to low temperature is an adaptability of rice (Oryza sativa L.) to cold. The mutant, a conditional expression controlled by development and temperature, differs from other albino mutants. The chlorophyll content of the mutant was measured using a portable chlorophyll meter, and the ultrastructure of the chloroplast was observed using a transmission electron microscope. Chlorophyll content was 1.2 SPAD, and the chloroplast did not develop, with only small vesicle-like structures. A segregation analysis of the reciprocal crosses between the albino mutation line with the rice line 9311 demonstrated that the albino trait was controlled by a single recessive gene, which was flanked by SSR markers RM5068 and RM3702 on the short arm of chromosome 8 with a distance of 0.5-1.1 cM and 4.9 cM, respectively. This gene was mapped within a 6 cM interval region and was tentatively referred to as al12. Key words: rice; albino mutant; chloroplast; gene mapping At present, many albino mutants have been versity adaptation of rice. By crossing and back- found in rice (Oryza sativa L