《Archaeal diversity and abundance within different layers of summer seaice and seawater from Prydz Bay Antarctica》.pdfVIP

•Article • Advances in Polar Science doi: 10.13679/j.advps.2014.1.00054 March 2014 Vol. 25 No. 1: 54-60 Archaeal diversity and abundance within different layers of summer sea-ice and seawater from Prydz Bay, Antarctica 1,2 2 1 1 1 1 MA Jifei , DU Zongjun , LUO Wei , YU Yong , ZENG Yixin , CHEN Bo LI Huirong1* 1 Polar Research Institute of China, Shanghai 200136, China; 2 College of Marine Science, Shandong University, Weihai, Weihai 264209, China Received 15 May 2013; accepted 30 October 2013 Abstract Fluorescence in-situ hybridization (FISH) and 16S rRNA gene clone library analyses were used to determine the abundance and diversity of archaea in Prydz Bay, Antarctica. Correlation analysis was also performed to assess links between physicochemical parameters and archaeal abundance and diversity within the sea-ice. Samples of sea-ice and seawater were collected during the 26th Chinese National Antarctic Research Expedition. The results of FISH showed that archaea were relatively abundant within the top layer of the sea-ice, and correlation analysis suggested that the concentration of NH + might be 4 one of the main factors underlying this distribution pattern. However, using 16S rRNA gene libraries, archaea were not detected in the top and middle layers of the sea-ice. All archaeal clones obtained from the bottom layer of the sea-ice were grouped into the Marine Group I Crenarchaeota while the arch