利用分叶顺次肝切除术建立小鼠肝脏大部切除后再生模型.pdfVIP

2009 5 305 http : / / ww w . aj smm u. cn 524 Academic Journal of Second Military M edical University, May 2009, Vol. 30, No. 5 OI: 10.3724/ SP.J. 1008. 2009.00524 论 著 1 2 3 3 1* 2* 1 汤朝晖, 周露婷 ,谢志芳 ,胡以平 ,杨甲梅 , 章卫平 , 吴孟超 1. , 200438 2. , 200433 3. , 200433 [ ] 目的: 利用肝部分切除术, 建立可准确量化和易于操控的小鼠肝脏再生模型, 为研究肝脏再生的 胞和分子生物学 机制及其病理学意义提供技术平台方法:正常成年C57BL/6 小鼠经心脏灌注固定后, 逐叶分离肝脏称重, 计算各肝叶所 占肝重的百分比; 麻醉状态下, 分叶顺次无菌切除小鼠肝脏的左外叶左中叶和右中叶, 建立肝脏大部切除的再生模型; 利用 RT- PCR 方法动态检测术后肝脏甲胎蛋白基因的激活表达特征结果: 肝左外叶左中叶和右中叶合计约占小鼠肝脏总重 量的68%,分叶顺次切除上述3 个肝叶, 术后动物恢复和存活状态良好, RT- PCR 结果证实甲胎蛋白基因在小鼠肝脏再生中 的激活表达结论: 利用分叶顺次肝切除术, 成功建立了小鼠的肝大部切除后再生模型,该方法可准确量化肝脏切除的程度, 具有可控性强简便易行和成功率高等优点, 为小鼠肝再生的研究奠定了基础 [ ] 肝解剖; 肝部分切除术; 肝再生 [ ] R 333. 4 [ ] A [ ] 0258- 879X(2009)05- 0524- 03 Establishment of mouse liver regeneration model by consecutive partial hepatectomy 1 2 3 3 1* 2* TANG Zhao- hui ,ZHOU Lu- ting , XIE Zhi- fang , HU Yi- ping , YANG Jia- mei , ZHANG Wei- ping , WU Meng- chao1 1. Eastern Hepatobiliary Hospital, Second Military Medical University,Shanghai 200438 2. epartment of Pathophysiology,College of Basic Medical Sciences, Second Military M edical University, Shanghai 200433 3. epartment of Cell Biology, College of Basic Medical Sciences, Second Military Medical U niversity,Shanghai 200433 [ ABSTRACT] Objective To establish a quantifiable and easily manipulable mouse model of liver regeneration by partial hepatectomy, so as to provide a basis for investigating the underlying cellular and molecular mechanisms and pathophysiological significance of liver regeneration. Methods Normal adult C57BL/ 6 mice were fixed by cardiac perfusion,the liver lobes were dissected and weighed, and the proport