白芍总苷的降血脂抗氧化作用及其对脂肪肝的防治研究.docVIP

白芍总苷的降血脂、抗氧化作用及其对脂肪肝的防治研究 　　作者：刘月丽1，吕俊华2　 作者单位：(1.海南医学院药学院药理教研室，海南 海口　571101; 2.暨南大学药学院药理教研室，广东 广州　510632) 　　【摘要】 目的：研究白芍总苷的降血脂、抗氧化作用及其对脂肪肝的防治作用。方法：采用高脂高糖饮食建立脂肪肝大鼠模型，并预防性给予白芍总苷处理8周。8周后，处死大鼠，测定血浆中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)水平;取全肝称重，计算肝指数，同时测定肝组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT) 和谷胱甘肽过氧化物酶(GSH-PX)活性以及丙二醛(MDA)的含量，取肝左叶，HE染色，光镜下观察肝脏组织学变化。结果：白芍总苷可明显降低脂肪肝大鼠血浆TC、TG、LDL-C水平，提高血浆HDL-C水平;降低肝指数，降低ALT和AST水平，明显提高肝脏组织中SOD、CAT 和GSH-PX活性，降低MDA含量，改善肝脏组织学改变。结论：白芍总苷对高脂高糖饮食诱导的脂肪肝有防治作用，其作用机制可能与降血脂和提高肝脏抗氧化能力有关。 　　【关键词】 白芍总苷;脂肪肝;降血脂;抗氧化;防治作用 　　[ABSTRACT] Objective： To study hypolipidemic and antioxidative effect and hepatic protective effect of total glucosides of paeony′s on non-alcoholic fatty liver. Methods：Rats were bred with high sugar and fat diet to establish non-alcoholic fatty liver model. Total glucosides of paeony ( 0.1 g/kg ) was administered to prevent development of non-alcoholic fatty liver. After 8 weeks， rats were sacrificed and the blood was collected to test total cholesterol (TC)， triglyceride(TG)， high density lipoprotein cholesterol(HDL-C)， low density lipoprotein cholesterol(LDL-C) levels and the activities of aspartate aminotransferase(AST)， alanine aminotransferase (ALT). Isolated liver was weighed to calculate liver index and used to evaluate the activities of glutathione peroxidase (GSH-PX)， superoxide dismutase (SOD)， catalase (CAT) and the amount of malonaldehyde (MDA) as well as histological changes. Results：Total glucosides of paeony can decrease TG， TC， LDL-C， increase HDL-C， lower the activities of ALT， AST and liver index of non-alcoholic fatty liver rats， in addition， total glucosides of paeony can enhance activities of SOD， CAT， GSH-PX， diminish amount of MDA greatly and improved histological changes in liver of non-alcoholic fatty liver rats. Conclusion：Total glucosides of paeony can decrease serum lipid and improve hepatic function of non-alcoholic fatty liver rats; furthermore， the anti-oxidative a