药用真菌双向性固体发酵对雷公藤减毒持效研究.docVIP

药用真菌双向性固体发酵对雷公藤减毒持效的研究( 庄毅，苏明声，谢小梅﹡﹡ （江西中医学院现代中药制剂教育部重点实验室菌物药研究中心，江西 南昌 330004） [摘 要] 目的：研究双向固体发酵后雷公藤菌质的急性毒性，对小鼠免疫功能、抑瘤作用和抗炎镇痛的作用。 方法：以改进寇氏法测定发酵后不同时间雷公藤菌质的LD50。以各雷公藤菌质的LD50的1/6量给小鼠连续灌胃7天后脱颈处死，取脾细胞培养，分别用ConA、LPS刺激，MTT法检测T/B淋巴细胞的增殖情况。采用小鼠肉瘤(S180)移植性肿瘤模型，观察灵雷菌质乙酸乙酯、正丁醇、石油醚和水提取物的肿瘤抑制作用。通过二甲苯致耳肿胀法观察雷菌质的抗炎作用；采用热板法观察雷菌质的镇痛作用。Key Laboratory of Modern? Preparation of TCM, Mycomedicine Research Center, Jiangxi Nanchang 330004) [Abstract] OBJECTIVE: To study of the toxicity ,the effect on immunity, anti-tumor, anti-inflammatory and analgesic of fungal fermentative products in Tripterygium wilfordii Hook(TWFFP) by a novel bi-directional solid-state fermentation. METHODS: Toxicity of TWFFP was with LD50, then mice were taken the durgs based on one-sixth of LD50 for 7 days and killed, spleen calls was cultured , and the method of MTT was used to detect the situation of proliferation both T and B lymphocytes. Mice bearing transplanted tumor S180 was used to survey the anti-tumor’s effect of extractive TWFFP, such as ethyl acetate part, n-butyl alcohol part, petroleun ether and water pater. And the mice’s thymus index and spleen index had been measured. Model of acute inflammatory reactions of xylene-induced mouse ear edema was performed to study the anti-inflammatory effects, and hot plate test in mice were used to study the analgesic effect. RESULT: Toxicity of TWFFP was sharply lower than the raw materials, and TWFFP had the inhabiting effect on proliferation both T and B lymphocytes. All extractive appeared significant tumor resistance, its resisting rate all above 35%,especially n-butyl alcohol part’s anti-rate is 68.88. TWFFP significantly suppressed xylene-induced mouse ear edema and pain threshold was remarkably improved. CONCLUSION Toxicity of TWFFP could be reduced by bi-direction solid fermentation and the effect of immunosuppression, anti-tumor anti-inflammatory and analgesic were maintaining. KEY WORD: bi-direction solid-state fermentation; fungal fermentive