bFGF与骨髓间充质干细胞联合移植对脑损伤后神经再生的影响.pdf

M ar. 2009 25(2) 129~ 134 (Chinese Journal ofNeuroanatomy) bFGF 黄兴锐 朱云霞 赵爱琴 刘海昌 夏春林* ( , 215123) ( bFGF)( BMSCs) BMSCs, Hoechst 3334240 4: BM SCsbFGF + BMSCs , 10, , 2 d caspase3 BMSCs ; 2 w H Y: bFGF+ BMSCs caspase3 BM SCs 0. 5; BM SCs bFGF+ BM SCs(P 0. 05), bFGF + BMSCs BMSCs(P 0. 05); GFAPMAP2, BM SCs, bFGF+ BMSCs, MAP2 bFGF+ BMSCs BM SCs The effect of FGF and onemesenchymal stem cell unite transplantation on neural regeneration in traumatic rain injury H uang X ing rui, Zhu Yunx ia, Zhao A iq in, L iuH a ichang, X ia Chunlin ( Box i Institute ofC linicalAnatomy CellularNeurobiology Lab, M edical College of Soochow University, Suzhou 215123) A stract To study the effect of basic fibroblast growth factor ( bFGF) and bonemesenchymal stem cells ( BMSCs) unite transplantation on neural regeneration after traumatic brain injury (TBI). BM SCswere obtained from mouseby using the serum culturing. BM SCs labled by Hoechst 33342 and bFGFwere transplanted by vena caudalis. Fourty m icewere divided randomly into four groups including normal con trol group, injured group, BM SCs transplantation group and bFGF+ BM SCs transplantation group ( n = 10 for each group). A fter 2 days, caspase3 labeled mi munoflurescencewas used to detect the apoptosis of BMSCs. A fter2 weeks, the infarction areawasmeasured by using H staining combined w ith mi age analysis system software; mi munohistochemistry was used to analyze the number of neurons and astro cytes; learning and memory abilitiesweremeasured by Ymaze test. The results showed that the c