不同卷烟接装纸中菌落总数的测定.pdfVIP

第26 卷第4 期 湖南文理学院学报( 自然科学版) Vol. 26 No. 4 2014 年12 月 Journal of Hunan University of Arts and Science(Natural Science Edition) Dec. 2014 doi: 10.3969/j.issn.1672-6146.2014.04.020 不同卷烟接装纸中菌落总数的测定 ∗ 张 亮 , 李小龙, 王 颖, 刘贤杰, 谢 涛 (深圳烟草工业有限责任公司 技术中心, 广东 深圳, 518109) 摘 要: 采用平板计数法测定了10种市售卷烟接装纸的菌落总数, 结果表明: (1) 平板计数法适用于卷烟接装 纸菌落总数的测定; (2) 平板计数法检测接装纸菌落数重复性较好, 同一稀释度(1:10)平行样检测结果的相对 标准偏差(RSD)小于30%, 同一样品平行样RSD 小于20%; (3) 不同接装纸样品差异性显著, 同一厂家接装纸 样品差异性不显著; (4) 所测接装纸中的菌落总数均符合行业限量标准, 可判定为合格. 关键词: 卷烟接装纸; 菌落总数; 平板计数法 中图分类号: TS 41+ 1 文章编号: 1672-6146(2014)04-0078-03 The determination of the total number of colonies on different tipping papers ZHANG Liang, LI XiaoLong, WANG Ying, LIU XianJie, XIE Tao (Technology Centre, Shenzhen Tobacco Co, Ltd, Shenzhen 518109, China) Abstract: The total number of colonies of 10 commercially available tipping papers was determined by the plate count method, and the results show that: (1) the plate count method is suitable for the determination of the total number of colonies of the tipping papers; (2) the reproducibility of the plate count method is relatively stable, the relative standard deviation(RSD) of the same dilution parallel (1:10) was below 30%, and the RSD of the same sample parallel is below 20%; (3) the difference between the different tipping papers is significant, while the difference among the tipping papers from the same manufacture is not; (4) the total number of colonies determined of all the 1