有关微生物的论文微生物检验论文.pdfVIP

3346 重庆医学2010 年12 月第39 卷第24 期 夏 云, 曹 何, 严 立, 张晓恒, 张莉萍 (重庆医科大学附属第一医院检验科 400016) : 评价临床微生物 内和 间革兰染色和抗酸染色显微镜检查的一致性 对同一标本进行革兰染色显 微镜检查以及采用CAP 质控片进行显微镜检查, 以评价同一实验 人员之间的一致性; 对通过ISO15189 质量认证的3 个实验 送检的革兰染色和抗酸染色比对片进行检查, 将结果与送检单位比较以考察不同实验 之间显微显微镜检查查的一致性 同一实验 人员显微镜检查 一致性为96. 7% , 与其他3 个实验 革兰染色显微镜检查 一致性分别为90% 85% 87% ; 抗酸染 色显微镜检查 一致性分别为90% 80% 100% 在严格实行 内质量控制程序和人员培训的实验 , 误差因素得到有效 的控制,显微镜检查具有很好的一致性, 实验 间的结果具有良好的可比性 :显微镜检查; 人员比对 doi: 10. 3969/j. issn.2010.23. 019 :R446.5 : A : 2010) Implementation and evaluation of consistency for microscopic examination in clinical microbiology X IA Yun, CA O H e, YA N L i, et al . ( Dep artment of Clinic L aboratory, the First Af f il iate H osp ital, Chong qing Me ical University , Chongqing 400016, China) Abstract: Objective o evaluate the consistency of Gram st ain and Acidfast stain microscopic examination in different stuff and different microbiological laboratories. Methods he same sample and the CAP control smears were Gram stained and micro scopic examined by our work staff. he results were used to evaluate the consistency of different person in our laboratory. he stained smears collected from three laboratories w hich authorized by ISO15189 w ere microscopic examinated and the consistency was evaluated by comparing the results of our and sample offering laboratory.Results In total six people w ere involved in evalua t ion,the consistencies of Gram stain microscopic examination w as 96. 7% . he consistency of Gram stain and Acidfast stain micro scopic examinat ion copared with t he first,the second and the third laboratory wree 90% , 85% , 87% and 90% , 80% , 100% respect ly. Conclusion In the laboratory that executing stringent quality insurance and staff training programe, er